EPO mimetic peptide-1

EPO mimetic peptide-1 (EMP-1), a 20 amino acid peptide. EPO mimetic peptide-1 stimulates cell proliferation, affects cell cycle and induces the production of reticulocytes in vivo[1].

Signaling Pathways >> Others >> Others

Research Areas >> Inflammation/Immunology

EPO mimetic peptide-1 shows inhibition of tracer 125I-labeled EPO binding with an IC50 value of 0.2 μM[1]. EPO mimetic peptide-1 (0-50 mM; 48 h) stimulates proliferation of both TF-1 and B6Sut cells in a dose-dependent manner[1]. EPO mimetic peptide-1 (1 and 10 μM) promots erythroid colony formation from human peripheral blood and murine bone marrow[1]. EPO mimetic peptide-1 (10 μM; 10 min) induces the phosphorylation of proteins[1]. EPO mimetic peptide-1 (1 and 10 μM; 0-18 h) affects cell cycle[1]. Western Blot Analysis[1] Cell Line: FDCP-l/mEPOR cell line Concentration: 10 μM Incubation Time: 10 min Result: Induced the phosphorylation of proteins with apparent molecular weights of 140, 95, 70, and 55 Kd. Cell Cycle Analysis[1] Cell Line: FDCP-l/mEPOR cell line Concentration: 10 μM Incubation Time: 0, 6, 8, 10, 12 and 18 h Result: Increased cell progression into S phase.

EPO mimetic peptide-1 (1 and 2 mg; once) induces the production of reticulocytes over the background of endogenous in normal, untreated mice containing endogenous basal levels of EPO mice[1].