triptohypol C

Triptohypol C, a Tripterin (HY-13067) derivative, is a potent Nur77-targeting anti-inflammatory agent with an Kd value of 0.87 μM. Triptohypol C inhibits inflammatory response by promoting the interactions of Nur77 with TRAF2 and p62/SQSTM1[1].

Signaling Pathways >> Others >> Others

Research Areas >> Inflammation/Immunology

Kd: 0.87 μM (Nur77)[1]

Triptohypol C (compound 3a) (2 μM; 1 h) strongly antagonize the effect of TNFα on inducing IκBα degradation, and inhibits inflammatory response by promoting the interactions of Nur77 with TRAF2 and p62/SQSTM1[1]. Triptohypol C (2 μM; 10 h) cause 3.12% apoptosis in HepG2 cells, which is less toxic than Tripterin[1]. Western Blot Analysis[1] Cell Line: Lysates from HepG2 cells (incubated with 20 ng/mL TNFα for 30 min) Concentration: 2 μM Incubation Time: 1 h Result: Strongly antagonized the effect of TNFα on inducing IκBα degradation Immunofluorescence[1] Cell Line: HepG2 cells (transfected with Myc-Nur77 and Flag-TRAF2 or Flag-p62) Concentration: 2 μM Incubation Time: 1 h Result: Promoted the interactions between Nur77 and TRAF2 and p62/SQSTM1. Apoptosis Analysis[1] Cell Line: HepG2 cells Concentration: 2 μM Incubation Time: 10 h Result: Caused 3.12% apoptosis in cells, which was less cytotoxic than Tripterin (>10%).

Caused 3.12% apoptosis in cells, which was less cytotoxic than Tripterin (>10%). Animal Model: Zebrafish[1] Dosage: 0.5 μM, 1 μM and 1.25 μM Administration: 72 h Result: Had less effect than Tripterin on the death rate and malformation of zebrafish either at a concentration of 1.25 μM for 24 h or at a concentration of 0.5 μM for 72 h.