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Ac-Leu-Glu-His-Asp-AFC trifluoroacetate salt

Names

[ CAS No. ]:
210345-03-2

[ Name ]:
Ac-Leu-Glu-His-Asp-AFC trifluoroacetate salt

[Synonym ]:
L-α-Asparagine, N-acetyl-L-leucyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-
Acetyl-Leu-Glu-His-Asp-AFC
Ac-LEHD-AFC
AFC154
PASE-9 SUBSTRATE,FLUOROGENIC
N-Acetyl-L-leucyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine
Ac-Leu-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin
Ac-Leu-Glu-His-Asp-AFC

Biological Activity

[Description]:

Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase[1].

[Related Catalog]:

Research Areas >> Others
Signaling Pathways >> Apoptosis >> Caspase

[Target]

Caspase-9[1]


[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The assessment of the caspase activity by fluorogenic substrate assay[1]: 1. Preparation of cell lysis buffer: 1 % Nonidet P-40, NaCl 200 mM, Tris/HCl 20 mM, pH 7.4, Leupeptin 10 mg/L, Aprotinin (HY-P0017) (0.27 kU/L) and 100 mM PMSF (HY-B0496) . 2. Incubate the lysis buffer (25 mg of total protein) with 50 mmol/L of AC-DEVD-AMC (HY-P1003) (caspase-3), AC-VEID-AFC (caspase-8) or AC-LEHD-AFC (caspase-9) in the buffer (HEPES 10 mM, pH 7.4, containing Mannitol 220 mM, Sucrose 68 mM, NaCl 2 mM, KH2PO4 2.5 mM, EGTA 0.5 mM, MgCl2 2 mM, Pyruvate 5 mM, PMSF 0.1 mM, and Dithiothreitol 1 mM). 3. Assess the caspase activity by measuring fluorescent 7-amino-4-methylcoumarin released for 1 h at a 2-min intervals by a spectrofluorometer.

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Boiling Point ]:
1211.9±65.0 °C at 760 mmHg

[ Molecular Formula ]:
C33H38F3N7O11

[ Molecular Weight ]:
765.690

[ Flash Point ]:
686.7±34.3 °C

[ Exact Mass ]:
765.258118

[ PSA ]:
278.99000

[ LogP ]:
1.45

[ Vapour Pressure ]:
0.0±0.3 mmHg at 25°C

[ Index of Refraction ]:
1.587

Related Compounds