ZT-1a
Names
[ CAS No. ]:
212135-62-1
[ Name ]:
ZT-1a
Biological Activity
[Description]:
ZT-1a is a potent, non-ATP-competitive and selective SPAK inhibitor. ZT-1a inhibits SPAK activity with IC50s of 44.3, 35.0, 46.7 μM at ATP concentrations of 0.01, 0.1 and 1 mM, respectively[1].
[Related Catalog]:
[Target]
SPAK[1]
[In Vitro]
ZT-1a inhibits Na-K-2Cl cotransporter (NKCC1) and stimulates K-Cl cotransporters (KCCs) by decreasing their SPS1-related proline/alanine-rich kinase (SPAK)-dependent phosphorylation[1]. ZT-1a inhibits phosphorylation of NKCC1 p-Thr203/207/212 by 72±5.2% at 1 µM ZT-1a and phosphorylation of KCC sites 1/2 by 65-77% at 3 µM in HEK-293 cells[1]. SPAK phosphorylation at Ser373 is inhibited by 70±3.8% inhibition at 3-10 µM ZT-1a[1]. ZT-1a (10 µM) inhibits NKCC1 but stimulates KCC3 activity[1].
[In Vivo]
ZT-1a (10-100 mg/kg) inhibits SPAK-dependent cation-Cl− cotransporters (CCC) phosphorylation in vivo[1]. Animal Model: Naive mice[1] Dosage: 10, 30, 50, and 100 mg/kg Administration: Intraperitoneal (i.p.) administration Result: Inhibited SPAK-dependent cation-Cl- cotransporters (CCC) phosphorylation in vivo.
[References]
Chemical & Physical Properties
[ Molecular Formula ]:
C22H15Cl3N2O2
[ Molecular Weight ]:
445.73