DiFMUP

Names

[ Name ]:
DiFMUP

[Synonym ]:
aurora ka-6718
difmup

Biological Activity

[Description]:

DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].

[References]

[1]. Gee KR, et, al. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal Biochem. 1999 Aug 15;273(1):41-8.

[2]. Welte S, et, al. 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal Biochem. 2005 Mar 1;338(1):32-8.

Chemical & Physical Properties

[ Molecular Formula ]:
C₁₀H₇F₂O₆P

[ Molecular Weight ]:
292.13000

[ Exact Mass ]:
291.99500

[ PSA ]:
106.78000

[ LogP ]:
1.85110

[ Storage condition ]:
-20°C

Safety Information

[ Safety Phrases ]:
22-24/25

[ HS Code ]:
2932209090

Customs

[ HS Code ]: 2932209090

[ Summary ]:
2932209090. other lactones. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%