Anticancer agent 32

Anticancer agent 32 (compound 2g) is an anticancer agent. Anticancer agent 32 shows anticancer activities, affects cell cycle and induces cell apoptosis. Anticancer agent 32 can be used for the research of cancer[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

IC50: 17.2 μM (MGC-803), 12.3 μM (HeLa), 40.6 μM (NCI-H460), 46.8 μM (HepG2), 95.4 μM (SMMC-7721), 8.9 μM (T-24), 86.8 μM (HL-7702)[1]

Anticancer agent 32 (2.5-40 μM; 48 h) shows cytotoxic activities to cancer cell lines[1]. Anticancer agent 32 (0-16 μM; 24 h) affects cell cycle, induces cell apoptosis, increases intracellular levels of Ca2+ and ROS[1]. Cell Cytotoxicity Assay[1] Cell Line: MGC-803, HeLa, NCI-H460, HepG2, SMMC-7721, T-24 and HL-7702 cell lines Concentration: 2.5, 5, 10, 20 and 40 μM Incubation Time: 48 hours Result: Inhibited cell growth with IC50 values of 17.2, 12.3, 40.6, 46.8, 95.4, 8.9 and 86.8 μM for MGC-803, HeLa, NCI-H460, HepG2, SMMC-7721, T-24 and HL-7702 cells, respectively. Cell Cycle Analysis[1] Cell Line: MGC-803 and T-24 cell lines Concentration: 0, 8 and 12 μM Incubation Time: 24 hours Result: Significantly induced cell cycle arrest at the G2/M phase in T-24 cells. Western Blot Analysis[1] Cell Line: MGC-803 and T-24 cell lines Concentration: 4, 8 and 12 μM Incubation Time: 24 hours Result: Decreased expression level of cyclin B1. Apoptosis Analysis[1] Cell Line: MGC-803 and T-24 cell lines Concentration: 0, 4, 8 and 16 μM Incubation Time: 24 hours Result: Induced cell apoptosis of T-24 cells from 8.21 to 32.91% after treatment by increasing levels of caspase-3 and caspase-9 in T-24 cells.

[1]. Li GZ, et al. Synthesis and biological evaluation of novel 1,3-diphenylurea quinoxaline derivatives as potent anticancer agents. Med Chem Res 30, 1496–1511 (2021).