Disodium 4-methyl-2-oxo-2H-chromen-7-yl phosphate

Names

[ Name ]:
Disodium 4-methyl-2-oxo-2H-chromen-7-yl phosphate

[Synonym ]:
Sodium 4-methyl-2-oxo-2H-chromen-7-yl phosphate
4-Methylumbelliferyl phosphate sodium salt
4-MUP,Disodium 4-methylumbelliferyl phosphate
EINECS 245-325-0
Disodium 4-methyl-2-oxo-2H-chromen-7-yl phosphate
2H-1-Benzopyran-2-one, 4-methyl-7-(phosphonooxy)-, sodium salt (1:2)
4-Methylumbelliferylphosphate disodium salt trihydrate
4-MUP
4-Methylumbelliferyl phosphate,disodium salt
MFCD00037614
Disodium 4-methylumbelliferyl phosphate

Biological Activity

[Description]:

4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant[1][2][3].

[Related Catalog]:

Signaling Pathways >> Metabolic Enzyme/Protease >> Phosphatase

Research Areas >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Serum acid phosphatase assay[1]: 1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0. 2. Add the following agents (with the final concentration) 20 mM Sodium Tartrate (HY-128476), 15 mM Sodium Fluoride (HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM 1, 10-Phenanthroline (HY-W004544), 6 mM 8-Quinolinol (HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mM EDTA (HY-Y0682) and 6 mM EGTA (HY-D0861). 3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5. 4. Mix samples thoroughly and determine fluorescence by a fluorometer.

[References]

[1]. Chambers JP, et al. Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. Clin Chim Acta. 1977 Oct 1;80(1):67-77.

[2]. Watanabe F, et al. The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane. Clin Chim Acta. 1979 Feb 1;91(3):273-6.

[3]. Hao Chen, et al. Autonomic Molecular Transport for Ultrasensitive Surface-Enhanced Infrared Absorption Spectroscopy. ACS Appl. Polym. Mater. 2020, 2, 9, 3929-3935

Chemical & Physical Properties

[ Boiling Point ]:
511.4ºC at 760 mmHg

[ Molecular Formula ]:
C₁₀H₇Na₂O₆P

[ Molecular Weight ]:
300.112

[ Flash Point ]:
263.1ºC

[ Exact Mass ]:
299.977570

[ PSA ]:
112.44000

[ LogP ]:
2.44930

[ Vapour Pressure ]:
2.79E-11mmHg at 25°C

[ Storage condition ]:
−20°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Risk Phrases ]:
22-24/25

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
2932209090

Customs

[ HS Code ]: 2932209090

[ Summary ]:
2932209090. other lactones. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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