Autophagy inducer 4

Autophagy inducer 4 is a Magnolol-based Mannich base derivatives, which can be used as an anticancer agent. Autophagy inducer 4 suppresses cancer cells via inducing autophagy. Autophagy inducer 4 has 76-fold improvement in cytotoxicity against T47D cells compared with Magnolol. Autophagy inducer 4 also possesses suppressive effects on migration of T47D and Hela cancer cells[1].

Research Areas >> Cancer

Signaling Pathways >> Autophagy >> Autophagy

IC50: 0.91 μM in T47D, 3.32 μM in MCF-7, 1.71 μM in Hela[1]

Autophagy inducer 4 (compound 3p) (0-10 μM; 72 hours) displays highly potent antiproliferative activity against T47D, MCF-7 and Hela cell lines[1]. Autophagy inducer 4 (40-80 μM; 0-36 hours) significantly increases GFP-LC3 protein puncta with both dose- and time-dependent manners in HEK293 cells[1]. Autophagy inducer 4 (0-80 μM; 0-36 hours) increases the transformation of LC3-I into LC3-II in a dose- and time-dependent manner[1]. Cell Proliferation Assay Cell Line: T47D, MCF-7 and Hela cells[1] Concentration: 0-10 μM Incubation Time: 72 hours Result: Displayed highly potent antiproliferative activity against T47D, MCF-7 and Hela cell lines with IC50 values of 0.91, 3.32 and 1.71 μM, respectively. Cell Autophagy Assay Cell Line: GFP-LC3-HEK293[1] Concentration: 40, 60 and 80 μM Incubation Time: 0, 12, 24 and 36 hours Result: Significantly increased GFP-LC3 protein puncta with both dose- and time-dependent manners in HEK293 cells. Western Blot Analysis Cell Line: Hela, T47D and HEK293[1] Concentration: 0, 5, 10 and 20 μM in T47D and Hela; 0, 40, 60 and 80 μM in HEK293 Incubation Time: 0, 12, 24 and 36 hours Result: Increased the transformation of LC3-I into LC3-II in a dose-dependent manner, and enhanced the expression of LC3-II in a time-dependent manner.

[1]. Xu T, et al. Semisynthesis of novel magnolol-based Mannich base derivatives that suppress cancer cells via inducing autophagy. Eur J Med Chem. 2020;205:112663.