MMP-9-IN-4

MMP-9-IN-4 is a MMP-9 inhibitor (IC50: 7.46 nM) that has H-π interactions with MMP-9. MMP-9-IN-4 also inhibits AKT activity (IC50: 8.82 nM). MMP-9-IN-4 shows cell cytotoxicity and induces cell apoptosis. MMP-9-IN-4 can be used in the research of cancers[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> PI3K/Akt/mTOR >> Akt

Signaling Pathways >> Metabolic Enzyme/Protease >> MMP

MMP-9:7.46 nM (IC50)

MMP-1:41 nM (IC50)

MMP-2:139 nM (IC50)

MMP-13:113 nM (IC50)

AKT:8.82 nM (IC50)

MMP-9-IN-4 (compound 11, 0-1 μM approximately, 72 h) shows cytotoxicity to Wi-38, MCF-7, NFS-60, HepG-2 cells[1]. MMP-9-IN-4 (72 h) induces apoptosis in MCF-7, NFS-60 and HepG-2 cells at 3.6 nM, 3.61 nM, 2.2 nM respectively[1]. MMP-9-IN-4 (72 h) induces significant caspase 3/7 activation in MCF-7, NFS-60 and HepG-2 cells at 3.6 nM, 3.61 nM, 2.2 nM respectively [1]. MMP-9-IN-4 (2.2 nM, 24 h) inhibits cell migration in HepG-2 cells[1]. MMP-9-IN-4 has anti-bacterial activity against E.coli with a MIC value of 1.814 μM[1]. Cell Viability Assay[1] Cell Line: Wi-38, MCF-7, NFS-60, HepG-2 cells Concentration: 0-1 μM approximately Incubation Time: 72 h Result: Showed cytotoxicity with IC50s: 374 nM (Wi-38), 3.6 nM (MCF-7), 3.61 nM (NFS-60), 2.2 nM (HepG-2). Cell Migration Assay [1] Cell Line: HepG-2 Concentration: 2.2 nM Incubation Time: 24 h Result: Inhibited cell migration by 93.77%.

[1]. Mohammed Salah Ayoup, et al. Battle tactics against MMP-9; discovery of novel non-hydroxamate MMP-9 inhibitors endowed with PI3K/AKT signaling attenuation and caspase 3/7 activation via Ugi bis-amide synthesis. Eur J Med Chem. 2020 Jan 15;186:111875.