fluorescein 5-isothiocyanate

Names

[ Name ]:
fluorescein 5-isothiocyanate

[Synonym ]:
5-FITC
Isothiocyanatofluorescein
5-Isothionatofluorescein
FITC isomer I
Fluorescein Isothiosyanate
fluorescein isothiocyanate isomer 1
FITC ISOMER
EINECS 222-042-0
3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofurane-1,9'-xanthén]-3-one
Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato-
FITC-I
Fluorescein 5(6)-isothiocyanate
FITC
Fluorescein-5-isothiocyanate
MFCD00005063
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-
Fluorescein Isothiocyanate
fluorescein 5-isothiocyanate
3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
5(6)-FITC

Biological Activity

[Description]:

5(6)-FITC (Fluorescein isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[References]

[1]. Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215-26.

[2]. Der-Balian GP, et al. Fluorescein labeling of Fab' while preserving single thiol. Anal Biochem. 1988 Aug 15;173(1):59-63.

[3]. Smith LM, et al. Fluorescence detection in automated DNA sequence analysis. Nature. 1986 Jun 12-18;321(6071):674-9.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Boiling Point ]:
708.6±60.0 °C at 760 mmHg

[ Melting Point ]:
>360ºC(lit.)

[ Molecular Formula ]:
C₂₁H₁₁NO₅S

[ Molecular Weight ]:
389.381

[ Flash Point ]:
382.4±32.9 °C

[ Exact Mass ]:
389.035797

[ PSA ]:
120.44000

[ LogP ]:
4.00

[ Appearance of Characters ]:
powder

[ Vapour Pressure ]:
0.0±2.3 mmHg at 25°C

[ Index of Refraction ]:
1.754

[ Storage condition ]:
2-8°C

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H334

[ Precautionary Statements ]:
P261-P342 + P311

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Faceshields;Gloves

[ Hazard Codes ]:
Xn: Harmful;

[ Risk Phrases ]:
R42/43

[ Safety Phrases ]:
S22

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

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Uptake of neutrophil-derived Ym1 protein distinguishes wound macrophages in the absence of interleukin-4 signaling in murine wound healing.

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The determination of regenerative wound-healing macrophages as alternatively activated macrophages is currently questioned by the absence of IL-4 in wound tissue. Yet, murine wound tissue expressed hi...