4',6-Diamidino-2-phenylindole dihydrochloride

Names

[ Name ]:
4',6-Diamidino-2-phenylindole dihydrochloride

[Synonym ]:
2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride
EINECS 249-186-7
DAPI hydrochloride
2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
MFCD00012681
2-(4-Amidinophenyl)
1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, hydrochloride (1:2)
4′,6′-Diamidino-2-phenylindole dihydrochloride
2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride
4',6-diamidine-2-phenylindole dihydrochloride
DAPI,Dihydrochloride
4,6-Diamidino-2-Phenylindole Dihydrochloride
DAPI dihydrochloride
4',6-Diamidino-2-phenylindole Dihydrochloride
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride

Biological Activity

[Description]:

DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

[In Vitro]

DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry[1].

[References]

[1]. Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.41g/cm3

[ Boiling Point ]:
545.4ºC at 760mmHg

[ Melting Point ]:
>300°C (dec.)

[ Molecular Formula ]:
C₁₆H₁₇Cl₂N₅

[ Molecular Weight ]:
350.246

[ Flash Point ]:
283.6ºC

[ Exact Mass ]:
349.086090

[ PSA ]:
115.53000

[ LogP ]:
5.60710

[ Vapour Pressure ]:
5.96E-12mmHg at 25°C

[ Storage condition ]:
−20°C

[ Water Solubility ]:
H2O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S26-S36-S24/25-S22

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
NL5995050

[ HS Code ]:
2933990090

Customs

[ HS Code ]: 2933990090

[ Summary ]:
2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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