Hexadimethrine bromide

Names

[ Name ]:
Hexadimethrine bromide

[Synonym ]:
Hexadimethrine bromide,Polybrene
HEXADIMETHRINE BROMIDE
1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide,Polybrene
MFCD00133397

Biological Activity

[Description]:

Hexadimethrine bromide is a cationic polymer discovered to enhance retroviral transduction.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Cancer

[In Vitro]

Hexadimethrine bromide inhibits human mesenchymal stem cell proliferation during lentiviral transduction. Hexadimethrine bromide is considered non-toxic at low concentrations, but has been found to negatively affect cell proliferation in some cell types at concentrations greater than 10 µg/mL. Trypsinized cells exposed to Hexadimethrine bromide are visibly larger in size when viewed under the microscope[1].

[Cell Assay]

Trypsinized cells are washed and resuspended in medium at 2×104 cells/mL with or without rhFGF-2 (final concentration=10 ng/mL, PeproTech) and with or without Hexadimethrine bromide at a final Hexadimethrine bromide concentration of 1, 4, or 8 µg/mL. The different conditions are seeded in 96-well plates at 1×103 cells in 50 µL per well in triplicate and cultured at 37°C, 5% CO2. After 6, 9, or 24 hr, the medium is changed and subsequent medium changes occurred every 3-4 days with 50 µL of ± FGF-2 medium (10 ng/mL). Plates are harvested on day 4, 7, 14, and 21 by removing the medium and placing the plates in the -80°C freezer until the day of analysis. The CyQUANT assay is then performed on the wells[1].

[References]

[1]. Lin P, et al. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction. PLoS One. 2011;6(8):e23891.

[Related Small Molecules]

Chemical & Physical Properties

[ Boiling Point ]:
209.5ºC at 760 mmHg

[ Molecular Formula ]:
C₁₅H₃₆Br₂N₂

[ Molecular Weight ]:
404.27

[ Flash Point ]:
73.9ºC

[ Vapour Pressure ]:
0.202mmHg at 25°C

[ Storage condition ]:
2-8℃

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: TQ2655000

CHEMICAL NAME :: Poly((dimethyliminio)hexamethylene(dimethylimino)trim ethylene dibromide)

CAS REGISTRY NUMBER :: 28728-55-4

LAST UPDATED :: 199703

DATA ITEMS CITED :: 4

WISWESSER LINE NOTATION :: /E3E &1N1&5N1&1/

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 20 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: USXXAM United States Patent Document. (U.S. Patent Office, Box 9, Washington, DC 20231) Volume(issue)/page/year: #4013507

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: >1 gm/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: USXXAM United States Patent Document. (U.S. Patent Office, Box 9, Washington, DC 20231) Volume(issue)/page/year: #4013507

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 30 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: RPTOAN Russian Pharmacology and Toxicology (English Translation). Translation of FATOAO. (Euromed Pub., 33, Woodlands Rd., Surbiton, Surrey, UK) V.30- 1967- Volume(issue)/page/year: 37,267,1974

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 28 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: USXXAM United States Patent Document. (U.S. Patent Office, Box 9, Washington, DC 20231) Volume(issue)/page/year: #4013507

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H302

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xn: Harmful;

[ Risk Phrases ]:
R20/21/22

[ RIDADR ]:
UN 2811

[ RTECS ]:
TQ2655000

[ Packaging Group ]:
III

[ Hazard Class ]:
6.1(b)

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Polybrene is widely used to enhance viral transduction; however, little is known about the utility thereof, in enhancing lentiviral transduction of cochlear cells. In the present study, we examined th...