Mca-(Ala7,Lys(Dnp)9)-Bradykinin trifluoroacetate salt

Names

[ Name ]:
Mca-(Ala7,Lys(Dnp)9)-Bradykinin trifluoroacetate salt

[Synonym ]:
7-methoxycoumarin-4-yl-acetyl-RPPGFSAFK-2,4-dinitrophenyl
McaBk2
MCA-ARG-PRO-PRO-GLY-PHE-SER-ALA-PHE-LYS(DNP)-OH
Mca-(Ala7,Lys(Dnp)9)-Bradykinin

Biological Activity

[Description]:

Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Mca-(ala7,lys(dnp)9)-bradykinin is also a substrate for neprilysin, but is hydrolyzed 10-fold more efficiently by ECE-1, making this substrate selective for ECE-1[1].

[References]

[1]. Johnson GD, et al. Development of an internally quenched fluorescent substrate selective for endothelin-converting enzyme-1. Anal Biochem. 2000 Nov 1;286(1):112-8.

Chemical & Physical Properties

[ Molecular Formula ]:
C₆₆H₈₁N₁₅O₁₉

[ Molecular Weight ]:
1388.44000

[ Exact Mass ]:
1387.58000

[ PSA ]:
506.86000

[ LogP ]:
5.21780