Bella Fluor 594 Phalloidin

Bella Fluor 594 Phalloidin is a photostable red-fluorescent probe with a high affinity for F-actin (Excitation/Emission=581/609 nm). Bella Fluor 594 Phalloidin can be used in cytoskeletal protein imaging[1][2].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). F-actin staining[2]: 1. Cells are pre-incubated with PBS containing 1% Bovine Serum Albumin (HY-D0842) for 20-30 min. 2. Staining with Bella Fluor 594 Phalloidin for 20 min. 3. Washing with PBS. 4. Using confocal laser-scanning for examination (Excitation/Emission=581/609 nm).

[1]. Lisa Leinhos, et al. Hypoxia suppresses myofibroblast differentiation by changing RhoA activity. J Cell Sci. 2019 Feb 18;132(5):jcs223230.

[2]. Sergiy Kostenko, et al. PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5. Cell Signal. 2009 May;21(5):712-8.