CaCCinh-A01
Names
Biological Activity
[Description]:
CaCCinh-A01 is an inhibitor of both TMEM16A and calcium-activated chloride channel (CaCC) with IC50s of 2.1 and 10 μM, respectively.
[Related Catalog]:
[Target]
IC50: 2.1 μM (TMEM16A)[1], 10 μM (CaCC)[2]
[In Vitro]
30 μM CaCCinh-A01 and 100 μM tannic acid strongly inhibit CaCC current following ATP stimulation[1]. Calcium-dependent chloride current is reduced by 38±14, 66±10, and 91±1% by 0.1, 1, and 10 μM CaCCinh-A01, respectively. ATP-induced short-circuit currents are reduced by 38±7 and 78±3% at 10 and 30 μM CaCCinh-A01, respectively[2].
[Cell Assay]
Each well of a 96-well plate is washed three times with PBS (200 μL/wash), leaving 50 μL of PBS. Test compounds (including CaCCinh-A01) (0.5 μL) are added to each well at 25 μM final concentration. After 10 min, 96-well plates are transferred to a plate reader for fluorescence assay. Each well is assayed individually for TMEM16A-mediated I- influx by recording fluorescence continuously (400 ms/point) for 2 s (base line), then 50 μL of a 140 mM I- solution containing 200 μM ATP is added. The initial rate of I- influx is computed from fluorescence data by nonlinear regression[1].
[References]
[Related Small Molecules]
Shikonine
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IAA-94
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Flufenamic Acid
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NPPB
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Lubiprostone
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Niflumic acid
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Adjudin
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Chlorotoxin TFA
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Fluxametamide
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Meticrane
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H100
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NS1652
Chemical & Physical Properties
[ Molecular Formula ]:
C18H21NO4S
[ Molecular Weight ]:
347.42900
[ Exact Mass ]:
347.11900
[ PSA ]:
107.78000
[ LogP ]:
4.51560
[ Storage condition ]:
-20℃
Safety Information
[ RIDADR ]:
NONH for all modes of transport