SC26304

SC26304 inhibits the effects of Aldosterone on urinary K+:Na+ ratios and the binding of [3H]Aldosterone to renal cytoplasmic and nuclear receptors.

Signaling Pathways >> Metabolic Enzyme/Protease >> Mineralocorticoid Receptor

Research Areas >> Metabolic Disease

Cytoplasmic binding of [3H]Aldosterone and [3H]SC-26304 is similar in magnitude and involves the same set of sites. Under three sets of conditions-(i) in the intact rat, (ii) in kidney slices, and (iii) in reconstitution studies (mixing prelabeled cytoplasm with either purified renal nuclei or chromatin), [3H]SC-26304 does not yield specific nuclear complexes in contrast to the reproducible generation of these complexes with [3H]Aldosterone. In glycerol density gradients, cytoplasmic [3H]Aldosterone receptor complexes sediment at 8.5 S and 4 S in low concentrations of salt and at 4.5 S in high concentrations of salt. Cytoplasmic [3H]SC-26304 receptor complexes sediment at 3 S in low concentrations of salt and 4 S in high concentrations of salt. These results are discussed in terms of an allosteric model of the receptor system. Administration of SC-26304 alone in doses of 3-600 μg/100 g of body weight has no effect on urinary Na+:creatinine or K+:creatinine ratios. These results are expressed as urinary K+:Na+ ratios. Aldosterone (0.3 μg/100 g of body weight) increases the K+:Na+ ratio 5-fold. This increase is significantly inhibited by 180 μg/100 g of body weight of SC-26304 and completely inhibit by 600 μg/100 g of body weight. To correlate inhibitory action and receptor occupancy, the same doses of SC-26304 are given to rats injected with 0.036 μg of [3H]Aldosterone. A dose of 180 μg of body weight reduces specific binding of Aldosterone in cytoplasmic and nuclear fractions to less than half of the control levels and 600 μg/100 g of body weight eliminated specific binding. The dose of Aldosterone used in the physiological studies is about eight times that used in the binding studies, but both doses are well below saturating amounts[1].

[1]. Marver D, et al. Renal aldosterone receptors: studies with (3H)aldosterone and the anti-mineralocorticoid (3H)spirolactone (SC-26304). Proc Natl Acad Sci U S A. 1974 Apr;71(4):1431-5.

Eplerenone | Esaxerenone | Fludrocortisone acetate | Deoxycorticosterone acetate | Canrenone | Osilodrostat | Apararenone