Safranin

Names

[ CAS No. ]:
477-73-6

[ Name ]:
Safranin

[Synonym ]:
Phenazinium, 3,7-diamino-2,8-dimethyl-5-phenyl-, chloride (1:1)
Safranin T
safranin A
C.I. Basic Red 2 (8CI)
Safranine
safranin Y
3,7-Diamino-2,8-dimethyl-5-phenylphenazin-5-ium chloride
Basic Red 2
Gossypimine
Safranine O,certified
Safranin
EINECS 207-518-8
Hidaco safranine
MFCD00011759
Safranine O
Safranin O
Tolusafranine
Safranine O,high purity biological stain
Cotton Red
Safranin Y or A
Safranine-O chloride
Safranine T
Basic Red 2,Cotton Red,Gossypimine

Biological Activity

[Description]:

Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. It can readily intercalate into biological macromolecules, including DNA and proteins. Safranin can be used as a redox indicator in the determination of metal ion concentration[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Wan H, et al. Structure characterization and optical properties investigation of the four main components of the classical phenazinium dye Safranin O. Analyst. 2019;144(24):7149-7156.

Chemical & Physical Properties

[ Density]:
1.00 g/mL at 20 °C

[ Molecular Formula ]:
C₂₀H₁₉ClN₄

[ Molecular Weight ]:
350.845

[ Flash Point ]:
46 °C

[ Exact Mass ]:
350.129822

[ PSA ]:
68.81000

[ LogP ]:
1.61230

[ Index of Refraction ]:
n20/D 1.339

[ Stability ]:
Stable.

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SG1623000

CHEMICAL NAME :: Phenazinium, 3,7-diamino-2,8-dimethyl-5-phenyl-, chloride

CAS REGISTRY NUMBER :: 477-73-6

LAST UPDATED :: 199701

DATA ITEMS CITED :: 11

MOLECULAR FORMULA :: C20-H19-N4.Cl

MOLECULAR WEIGHT :: 350.88

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 28740 ug/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 1600 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 24020 ug/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 26940 ug/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes

MUTATION DATA

TYPE OF TEST :: DNA repair

TEST SYSTEM :: Bacteria - Bacillus subtilis

DOSE/DURATION :: 2 mg/disc

REFERENCE :: TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho. Annual Report of Tokyo Metropolitan Research Laboratory of Public Health. (Tokyo-toritsu Eisei Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1- 1949/50- Volume(issue)/page/year: 27,153,1976 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1441 No. of Facilities: 1027 (estimated) No. of Industries: 9 No. of Occupations: 18 No. of Employees: 4314 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1441 No. of Facilities: 1253 (estimated) No. of Industries: 6 No. of Occupations: 10 No. of Employees: 22263 (estimated) No. of Female Employees: 14330 (estimated)

Safety Information

[ Symbol ]:

GHS05

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H318

[ Precautionary Statements ]:
P280-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi:Irritant;

[ Risk Phrases ]:
R36/38

[ Safety Phrases ]:
26-39-24/25-36-16

[ RIDADR ]:
UN 1170 3/PG 3

[ WGK Germany ]:
3

[ RTECS ]:
SG1623000

[ HS Code ]:
32041300

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2933990090

[ Summary ]:
2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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