Ninhydrin

Names

[ CAS No. ]:
485-47-2

[ Name ]:
Ninhydrin

[Synonym ]:
2,2-Dihydroxy-1H-indene-1,3(2H)-dione
Triketohydrinden Hydrate
Ninhydrin
2,2-Dihydroxy-1,3-indanedione
Ninhydrin,spectrophotometric grade
MFCD00003791
Ninhydrin Spray
Ningidrin
indane-1,2,3-trione monohydrate
NyNhydriN
NinhydrinGr
1,2,3-Indanetrione monohydrate
Trioxohydrindene monohydrate
Ninhydrin,ozone friendly ready to use spray
1H-Indene-1,2,3-trione hydrate
Ninhydrin (contains Acetic Acid) Ethanol
1,2,3-Indantrione, 2-hydrate
EINECS 207-618-1
NINHYDRINE
1,2,3-triketohydrindene hydrate
1H-Indene-1,3(2H)-dione, 2,2-dihydroxy-
2,2-dihydroxy-2H-indene-1,3-dione
Ninhydrin hydrate
NinhydrinA.R.
NIN
Ninthydrin
1,3-Indandione, 2,2-dihydroxy-
1,2,3-Indantrione monohydrate
1,2,3-Indantrione monohydrate,2,2-Dihydroxy-1,3-indanedione,Trioxohydrindene monohydrate

Biological Activity

[Description]:

Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins.

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[Target]

IC50: chromogenic analytical probe[1]

[In Vitro]

Ninhydrin is used in amino acid analysis of proteins. Except proline, Most amino acids can be hydrolyzed and react with ninhydrin. The amino acids are then quantified colorimetrically after separation by chromatography. Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product: diketohydrindylidene-diketohydrindamine.

[References]

[1]. Omar MA, et al. Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma.Luminescence. 2018 Sep;33(6):1107-1112.

[2]. Anantharaman S, et al. Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins.rSpectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 15;173:897-903.

Chemical & Physical Properties

[ Density]:
1.7±0.1 g/cm3

[ Boiling Point ]:
449.3±45.0 °C at 760 mmHg

[ Melting Point ]:
250 °C (dec.)(lit.)

[ Molecular Formula ]:
C₉H₆O₄

[ Molecular Weight ]:
178.141

[ Flash Point ]:
239.7±25.2 °C

[ Exact Mass ]:
178.026611

[ PSA ]:
74.60000

[ LogP ]:
1.07

[ Vapour Pressure ]:
0.0±1.2 mmHg at 25°C

[ Index of Refraction ]:
1.737

[ Storage condition ]:
Store at RT.

[ Stability ]:
Stable. Incompatible with amines, alkalies, strong bases. Light-sensitive.

[ Water Solubility ]:
0.1-0.5 g/100 mL at 20 ºC

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: NK5425000

CHEMICAL NAME :: 1,3-Indandione, 2,2-dihydroxy-

CAS REGISTRY NUMBER :: 485-47-2

BEILSTEIN REFERENCE NO. :: 1910963

LAST UPDATED :: 199701

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C9-H6-O4

MOLECULAR WEIGHT :: 178.15

WISWESSER LINE NOTATION :: L56 BV DV CHJ CQ CQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 250 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: NCNSA6 National Academy of Sciences, National Research Council, Chemical-Biological Coordination Center, Review. (Washington, DC) Volume(issue)/page/year: 5,28,1953

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 78 mg/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Vascular - regional or general arteriolar or venous dilation Lungs, Thorax, or Respiration - dyspnea

REFERENCE :: CRSBAW Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales. (SPPIF, B.P.22, F-41353 Vineuil, France) V.1- 1849- Volume(issue)/page/year: 151,719,1957 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - A1175 No. of Facilities: 33 (estimated) No. of Industries: 1 No. of Occupations: 2 No. of Employees: 594 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - A1175 No. of Facilities: 476 (estimated) No. of Industries: 4 No. of Occupations: 9 No. of Employees: 5565 (estimated) No. of Female Employees: 4002 (estimated)

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H302-H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xn:Harmful

[ Risk Phrases ]:
R22;R36/37/38

[ Safety Phrases ]:
S26-S28A-S45-S16

[ RIDADR ]:
UN 1170 3/PG 2

[ WGK Germany ]:
3

[ RTECS ]:
NK5425000

[ Hazard Class ]:
3.0

[ HS Code ]:
2915900090

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2915900090

[ Summary ]:
2915900090 other saturated acyclic monocarboxylic acids and their anhydrides, halides, peroxides and peroxyacids; their halogenated, sulphonated, nitrated or nitrosated derivatives VAT:17.0% Tax rebate rate:9.0% Supervision conditions:AB(certificate of inspection for goods inward,certificate of inspection for goods outward) MFN tariff:5.5% General tariff:30.0%

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