Rubropunctatin
Names
Biological Activity
[Description]:
Rubropunctatin, an orange azaphilone pigment, is isolated from the extracts of Monascus pilosus-fermented rice (red-mold rice). Rubropunctatin has anti-inflammatory, immunosuppressive and antioxidative effects, and also exhibits anti-tumor activity[1][2][3].
[Related Catalog]:
[In Vitro]
Rubropunctatin (1.5-30 μM; 24 h) shows selective tumoricidal effect on the human gastric carcinoma BGC-823 cells and no significant toxicity toward normal epithelial cell GES-1[1]. Rubropunctatin (5-30 μM; 6-24 h) induces apoptosis in a dose- and time-dependent manner in BGC-823 cells[1]. Rubropunctatin (0.75-8.0 µg/ml) exhibits DDPH radical scavenging activity, inhibition of super oxide radical generation and ferric reducing activity[2]. Cell Viability Assay[1] Cell Line: BGC-823 and GES-1cells Concentration: 1.5, 3, 6, 12, 15, 18, 30 μM Incubation Time: 24 hours Result: Decreased the viability of BGC-823 cells with an IC50 of 12.57 μM for 24 h. Did not show obvious cytotoxic effects on the normal cells. Cell Cycle Analysis[1] Cell Line: BGC-823 cells Concentration: 0, 5, 10, 30 μM Incubation Time: 0, 6, 12, 24 hours Result: Increased the percentage of cells in sub-G1 phase in a dose- and time-dependent manner.
[In Vivo]
Rubropunctatin (8-32 mg/kg; i.v. for 5 times) has anti-tumor effect mice[1]. Rubropunctamine inhibits 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice, with an ID50 of 0.11 mg/ear[3]. Animal Model: Male nude mice (5 weeks) are inoculated with BGC-823 cells[1] Dosage: 8, 32 mg/kg Administration: I.v. five times (day 1st, 4th, 7th, 10th, and 13th) Result: Diminished the tumor volume by 11.1% (8 mg/kg) and 24.2% (32 mg/kg). Reduced the tumor weight by 23.5% (8 mg/kg) and 37.7% (32 mg/kg). No significant difference was observed on the body weight.
[References]
Chemical & Physical Properties
[ Density]:
1.23g/cm3
[ Boiling Point ]:
638.8ºC at 760 mmHg
[ Molecular Formula ]:
C21H22O5
[ Molecular Weight ]:
354.39600
[ Flash Point ]:
280ºC
[ Exact Mass ]:
354.14700
[ PSA ]:
69.67000
[ LogP ]:
3.63110
[ Index of Refraction ]:
1.58