Dabsyl chloride

Names

[ Name ]:
Dabsyl chloride

[Synonym ]:
EINECS 260-235-1
4-[[4-(dimethylamino)phenyl]diazenyl]benzenesulfonyl chloride
MFCD00007444

Biological Activity

[Description]:

Dabsyl chloride is an amine derivatizing agent, able to give rise to stable products that can be easily monitored spectrophotometrically at 460 nm; Dabsyl chloride also used for labeling amino acids.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

Dabsyl chloride can give rise to mono-Dabsyl and bis-Dabsyl derivatives in the presence of multiple amino groups. Furthermore with respect to OPA derivatization, Dabsyl chloride can react with primary and also with secondary amines[1].

[Cell Assay]

Selected mouse brain samples from either cortical or striatal regions (100 mg wet weight) and neuroblastoma cells (SH-SY5Y) pellet derived from 25 cm2 flask are treated with 500 μL of 0.1 M HCl containing 0.2% TDGA, sonicated for 10 min (only for brain tissue), and then centrifuged at 14000 g for 30 min. The supernatant is freeze-dried. 50 μL of reaction buffer and 100 μL of 15 mM Dabsyl chloride are added to the tube and derivatized.

[References]

[1]. Francioso A, et al. HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens. Adv Exp Med Biol. 2017;975:535-549.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.29 g/cm3

[ Boiling Point ]:
483.6ºC at 760 mmHg

[ Melting Point ]:
185 °C (dec.)(lit.)

[ Molecular Formula ]:
C₁₄H₁₄ClN₃O₂S

[ Molecular Weight ]:
323.79800

[ Flash Point ]:
246.3ºC

[ Exact Mass ]:
323.05000

[ PSA ]:
70.48000

[ LogP ]:
5.17630

[ Index of Refraction ]:
1.607

[ Storage condition ]:
−20°C

[ Water Solubility ]:
DMF: soluble

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DB8927800

CHEMICAL NAME :: Benzenesulfonyl chloride, p-((p-(dimethylamino)phenyl)azo)-

CAS REGISTRY NUMBER :: 56512-49-3

LAST UPDATED :: 199612

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C14-H14-Cl-N3-O2-S

MOLECULAR WEIGHT :: 323.82

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: Unscheduled DNA synthesis

TEST SYSTEM :: Rodent - hamster Liver

DOSE/DURATION :: 10 umol/L

REFERENCE :: ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 5,1,1983

Safety Information

[ Symbol ]:

GHS05

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H314

[ Precautionary Statements ]:
P280-P305 + P351 + P338-P310

[ Personal Protective Equipment ]:
Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges

[ Hazard Codes ]:
C

[ Risk Phrases ]:
R22;R34

[ Safety Phrases ]:
S26-S36/37/39-S45-S27

[ RIDADR ]:
UN 3261 8/PG 2

[ WGK Germany ]:
3

[ RTECS ]:
DB8927800

[ Packaging Group ]:
II

[ Hazard Class ]:
8

[ HS Code ]:
2927000090

Customs

[ HS Code ]: 2927000090

[ Summary ]:
2927000090 other diazo-, azo- or azoxy-compounds。Supervision conditions:None。VAT:17.0%。Tax rebate rate:9.0%。MFN tariff:6.5%。General tariff:30.0%

Articles

A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry.

J. Mass Spectrom. 49(8) , 665-73, (2014)

Imaging mass spectrometry (IMS) studies increasingly focus on endogenous small molecular weight metabolites and consequently bring special analytical challenges. Since analytical tissue blanks do not ...

Lysine-directed staining of proteins for MS-based analyses.

Electrophoresis 34(3) , 401-4, (2013)

Visualization of proteins and MS-based analyses are elemental tasks in modern biochemistry. Nevertheless, reports about covalent protein dyes and their suitability for subsequent MS experiments remain...

Spectrophotometric determination of bacitracin in bulk drug as dabsyl derivative in a range of visible light.

Acta Pol. Pharm. 68(6) , 853-8, (2011)

A fast spectrophotometric method has been developed for bacitracin identification and determination after condensation reaction with dabsyl chloride. In addition, determination of dye stability of sul...