4',5'-DIBROMOFLUORESCEIN

Names

[ CAS No. ]:
596-03-2

[ Name ]:
4',5'-DIBROMOFLUORESCEIN

[Synonym ]:
MFCD00005042
EINECS 209-876-0
4',5'-dibromo-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one

Biological Activity

[Description]:

4',5'-Dibromofluorescein (Eosinic acid) is a fluorescein derivative that can used as a ligand for studying of protein properties by spectral analysis[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Dongdong Yu, et al. An ultrasensitive stain for negative protein detection in SDS-PAGE via 4',5'-Dibromofluorescein. J Proteomics. 2017 Aug 8;165:21-25.

Chemical & Physical Properties

[ Density]:
2.1 g/cm3

[ Boiling Point ]:
633.7ºC at 760 mmHg

[ Melting Point ]:
270-273 °C(lit.)

[ Molecular Formula ]:
C₂₀H₁₀Br₂O₅

[ Molecular Weight ]:
490.09800

[ Flash Point ]:
337ºC

[ Exact Mass ]:
487.88900

[ PSA ]:
75.99000

[ LogP ]:
5.19080

[ Index of Refraction ]:
1.771

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: LM5200000

CHEMICAL NAME :: Fluorescein, 4',5'-dibromo-

CAS REGISTRY NUMBER :: 596-03-2

BEILSTEIN REFERENCE NO. :: 0057189

LAST UPDATED :: 199710

DATA ITEMS CITED :: 6

MOLECULAR FORMULA :: C20-H10-Br2-O5

MOLECULAR WEIGHT :: 490.12

WISWESSER LINE NOTATION :: T C666 BO EVJ DE IR BVQ& MQ NE

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 15556 mg/kg/21D-C

TOXIC EFFECTS :: Kidney, Ureter, Bladder - other changes in urine composition Blood - changes in serum composition (e.g. TP, bilirubin, cholesterol) Related to Chronic Data - changes in ovarian weight

MUTATION DATA

TYPE OF TEST :: Mutation in microorganisms

TEST SYSTEM :: Bacteria - Salmonella typhimurium

DOSE/DURATION :: 100 ng/plate

REFERENCE :: ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 1,116,1979 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - T1904 No. of Facilities: 237 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 6161 (estimated) No. of Female Employees: 474 (estimated)

Safety Information

[ Symbol ]:

GHS06

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H301

[ Precautionary Statements ]:
P301 + P310

[ Personal Protective Equipment ]:
Eyeshields;Faceshields;Gloves;type P2 (EN 143) respirator cartridges

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36

[ Safety Phrases ]:
S22-S24/25-S45

[ RIDADR ]:
UN 2811 6.1/PG 3

[ WGK Germany ]:
3

[ RTECS ]:
LM5200000

[ HS Code ]:
2932999099

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2932999099

[ Summary ]:
2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%