Sulforhodamine 101

Names

[ Name ]:
Sulforhodamine 101

[Synonym ]:
sulforhodamine
MFCD00012407
Sulforhodamine-101
EINECS 262-159-4
Texas Red,Sulforhodamine 101 Acid Chloride
2-(2,3,6,7,12,13,16,17-octahydro-1h,5h,11h,15h-pyrido[3,2,1-ij]quinolizino[1',9':6,7,8]chromeno[2,3-f]quinolin-4-ium-9-yl)-5-sulfobenzenesulfonate

Biological Activity

[Description]:

Sulforhodamine 101 is a red fluorescent dye.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Neurological Disease

Dye Reagents

[In Vitro]

Sulforhodamine 101 does not label astrocytes in brainstem slices as strong and specific as in the hippocampus or cortex. To minimize excitatory side effects, the concentration of Sulforhodamine 101 has to be kept as low as possible or the labeling procedure can be performed after the actual experiment[1].

[In Vivo]

In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Sulforhodamine 101 or topical application of 100 μM[1].

[Cell Assay]

Acute brain slices are usually incubated in carbonated extracellular solution containing 0.5 to1 μM Sulforhodamine 101 for 20 to 30 min and 34 to 37°C. Following this, excess dye is removed over a period of 10 to 30 min using different protocols that were described earlier[1].

[Animal admin]

For in vivo imaging, Sulforhodamine 101 (SR101) is applied topically at concentrations of 250 nM to 300 μM or by bolus injection. Additionally, Sulforhodamine 101 injection over the tail vein (10 mg/mL) has been reported to be successful[1].

[References]

[1]. Hülsmann S, et al. Limitations of Sulforhodamine 101 for Brain Imaging. Front Cell Neurosci. 2017 Feb 28;11:44.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.1982 (rough estimate)

[ Molecular Formula ]:
C₃₁H₃₀N₂O₇S₂

[ Molecular Weight ]:
606.70900

[ Exact Mass ]:
606.14900

[ PSA ]:
143.81000

[ LogP ]:
4.59910

[ Index of Refraction ]:
1.6900 (estimate)

[ Water Solubility ]:
methanol: soluble1mg/mL

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
32049090

Precursor & DownStream

Precursor

DownStream

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