Brilliant blue G-250

Names

[ Name ]:
Brilliant blue G-250

[Synonym ]:
CBB G-250
benzenemethanaminium, N-[(1Z,4E)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
Coomassie Brilliant Blue G250
Benzenemethanaminium, N-[(1E,4E)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
EINECS 228-058-4
Cyanine G
benzenemethanaminium, N-[(1E,4Z)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
PAGE BLUE G90
coomassie briliant blue G-250
coomassie blue G-250
Sodium 3-({[(1E,4Z)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methyl-2,5-cyclohexadien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate
Coomassie Brilliant blue
Polar Blue G
Blue G 250
BRILLIANT BLUE
Sodium 3-({[(1Z,4E)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate
coomassie brillant blue G
SERVA BLUE G
MFCD00078482
Sodium 3-({[(1E,4E)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methyl-2,5-cyclohexadien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate

Biological Activity

[Description]:

Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome[1][2][3].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009;(30):1431. Published 2009 Aug 3.

[2]. Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82(11):4325-4328.

[3]. Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723.

Chemical & Physical Properties

[ Density]:
>1.0 g/cm3 (20ºC)

[ Melting Point ]:
100 °C

[ Molecular Formula ]:
C₄₇H₄₈N₃NaO₇S₂

[ Molecular Weight ]:
854.020

[ Flash Point ]:
11 °C

[ Exact Mass ]:
853.283142

[ PSA ]:
158.67000

[ LogP ]:
11.19820

[ Storage condition ]:
Store at RT.

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS02, GHS06, GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H225-H301-H311-H315-H319-H331-H370

[ Precautionary Statements ]:
P210-P260-P280-P301 + P310-P305 + P351 + P338-P311

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
F,T,Xn

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S22-S24/25-S36-S26-S45-S36/37/39-S16-S9-S7

[ RIDADR ]:
UN 2924 3/PG 2

[ WGK Germany ]:
3

[ HS Code ]:
32041200

Customs

[ HS Code ]: 32041200

Articles

Contrast recognizability during brilliant blue G - and heavier-than-water brilliant blue G-assisted chromovitrectomy: a quantitative analysis.

Acta Ophthalmol. 91(2) , e120-4, (2013)

To evaluate the potential of heavier-than-water brilliant blue G (BBG-D(2) 0) to stain the internal limiting membrane (ILM) during chromovitrectomy.In a nonrandomized, prospective, clinical multicentr...

Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones.

Appl. Environ. Microbiol. 75 , 1703-16, (2009)

SdiA is a homolog of quorum-sensing regulators that detects N-acylhomoserine lactone (AHL) signals from other bacteria. Escherichia coli uses SdiA to reduce its biofilm formation in the presence of bo...

Lipopolysaccharide inhibits the channel activity of the P2X7 receptor.

Mediators Inflamm. 2011 , 152625, (2011)

The purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. The bacterial endotoxin lipo...