Woodtide

Woodtide is a substrate for the DYRK (DYRK) family of kinases whose sequence is based on that around the DYRK phosphorylation site in FKHR[1][2].

Signaling Pathways >> Protein Tyrosine Kinase/RTK >> DYRK

Research Areas >> Neurological Disease

Immunoprecipitated FLAG-dDYRK2 or FLAG-MNB is assayed using 50 μM Woodtide or 50 μM activation-loop peptide with two additional lysines attached to the N terminus to allow it to bind to P81 paper (KKSSCYVDRKIYTYIQSRFY for dDYRK2 or KKSSCQLGQRIYHYIQSRFY for MNB). Both kinases efficiently phosphorylated the synthetic exogenous substrate peptide Woodtide, but neither dDYRK2 nor MNB phosphorylated their activation-loop peptide[1].

[1]. Pamela A Lochhead, et al. Activation-loop autophosphorylation is mediated by a novel transitional intermediate form of DYRKs. Cell. 2005 Jun 17;121(6):925-36.  

[2]. Linda E Campbell, et al. Differing substrate specificities of members of the DYRK family of arginine-directed protein kinases. FEBS Lett. 2002 Jan 2;510(1-2):31-6.