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Kemptide

Names

[ CAS No. ]:
65189-71-1

[ Name ]:
Kemptide

[Synonym ]:
L-leucyl-N-(diaminomethylidene)-L-ornithyl-N-(diaminomethylidene)-L-ornithyl-L-alanyl-L-seryl-L-leucylglycine
kemptide
Glycine, N-(N-(N-(N-(N2-(N2-L-leucyl-L-arginyl)-L-arginyl)-L-alanyl)-L-seryl)-L-leucyl)-
Glycine, L-leucyl-N-(diaminomethylene)-L-ornithyl-N-(diaminomethylene)-L-ornithyl-L-alanyl-L-seryl-L-leucyl-
L-Leucyl-N-(diaminomethylene)-L-ornithyl-N-(diaminomethylene)-L-ornithyl-L-alanyl-L-seryl-L-leucylglycine
MFCD00076710
LRRASLG

Biological Activity

[Description]:

Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).

[Related Catalog]:

Signaling Pathways >> Protein Tyrosine Kinase/RTK >> PKA
Signaling Pathways >> Stem Cell/Wnt >> PKA
Research Areas >> Metabolic Disease
Peptides

[In Vitro]

Kemptide is a synthetic construct of a substrate for cAMP-dependent protein kinase (PK). Different types of intact cells catalyze the phosphorylation of Kemptide in the presence of extracellular ATP and cAMP with Km values of 3-4 uM for Kemptide[1]. Kemptide is a synthetic peptide substrate derived from pyruvate kinase[2].

[Kinase Assay]

PKA activity is measured in pools of 10 oocytes at various stages of maturation using the SignaTECT cAMP-Dependent Protein Kinase (PKA) Assay System. Measurements of basal PKA activity are performed in the absence of exogenous cAMP, whereas measurements of total (cAMP-stimulated) PKA activity are performed in reaction buffer containing 0.025 mM cAMP[2].

[References]

[1]. Kübler D, et al. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55.

[2]. Duncan FE, et al. Transducin-like enhancer of split-6 (TLE6) is a substrate of protein kinase A activity during mouse oocyte maturation. Biol Reprod. 2014 Mar 20;90(3):63.


[Related Small Molecules]

Staurosporine | H 89 2HCl | Fasudil Hydrochloride | Bucladesine (sodium salt) | AT7867 | CCG215022 | HA-100 | AT13148 | Synaptamide | Warangalone | CREBtide | Ivachtin

Chemical & Physical Properties

[ Density]:
1.4±0.1 g/cm3

[ Molecular Formula ]:
C32H61N13O9

[ Molecular Weight ]:
771.908

[ Exact Mass ]:
771.471497

[ PSA ]:
381.95000

[ LogP ]:
-1.39

[ Index of Refraction ]:
1.626

[ Storage condition ]:
-20°C

MSDS

Click to get detail MSDS

Safety Information

[ Hazard Codes ]:
Xn

[ Safety Phrases ]:
S22-S24/25

[ WGK Germany ]:
3.0

Related Compounds