EGTA

Names

[ CAS No. ]:
67-42-5

[ Name ]:
EGTA

[Synonym ]:
Egtazic acid
ethylene glycol bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid
Egtazic
4F 4PP oxalate
CHEL-DE
Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid
EGTA
AEGT
ethylene glycol bis(2-aminoethyl)tetraacetic acid
(Ethylenebis(oxyethylenenitrilo))tetraacetic acid
Ethylene glycol bis(2-aminoethyl ether)tetraacetic acid
EINECS 200-651-2
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid
Ethylenebis(oxyethylenenitrilo)tetraacetic acid
MFCD00004291
3,12-Bis(carboxymethyl)-6,9-dioxa-3,12-diazatetradecane-1,14-dioic acid
chel tm-de
GIETA
[ethylenebis(oxyethylenenitrilo)]tetraacetic acid
6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-
GEDTA
ebonta
3,6-dioxaoctamethylenedinitrilotetraacetic acid

Biological Activity

[Description]:

EGTA is a specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA significantly inhibited the substrate adherence capacity of inflammatory macrophages[1][2].

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Inflammation/Immunology

[In Vitro]

EGTA, proposed as endodontic irrigant, decreases substrate adherence capacity of inflammatory macrophages in a time- and dose-dependent manner. The EGTA concentration that causes an IC50 is 202 mM. Chelators react with calcium ions in the hydroxyapatite crystals to produce a metallic chelate. Removal of calcium ions from the dentine makes the dentinal tissue softer, especially the hydroxyapatite-rich peritubular dentin and increases the diameter of exposed dentinal tubules[2].

[References]

[1]. Harris RA, Hanrahan JW. Effects of EGTA on calcium signaling in airway epithelial cells. Am J Physiol. 1994;267(5 Pt 1):C1426-C1434. doi:10.1152/ajpcell.1994.267.5.C1426

[2]. Segura-Egea JJ, Jiménez-Rubio A, Rios-Santos JV, Velasco-Ortega E, Calvo-Gutierrez JR. In vitro inhibitory effect of EGTA on macrophage adhesion: endodontic implications. J Endod. 2003;29(3):211-213.

Chemical & Physical Properties

[ Density]:
1.4±0.1 g/cm3

[ Boiling Point ]:
678.0±55.0 °C at 760 mmHg

[ Melting Point ]:
241 °C (dec.)(lit.)

[ Molecular Formula ]:
C₁₄H₂₄N₂O₁₀

[ Molecular Weight ]:
380.348

[ Flash Point ]:
363.9±31.5 °C

[ Exact Mass ]:
380.143097

[ PSA ]:
174.14000

[ LogP ]:
-2.53

[ Vapour Pressure ]:
0.0±4.5 mmHg at 25°C

[ Index of Refraction ]:
1.547

[ Storage condition ]:
Store at RT.

[ Water Solubility ]:
slightly soluble

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: AH3760000

CHEMICAL NAME :: Acetic acid, (ethylenebis(oxyethylenenitrilo))tetra-

CAS REGISTRY NUMBER :: 67-42-5

BEILSTEIN REFERENCE NO. :: 1717370

LAST UPDATED :: 199701

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C14-H24-N2-O10

MOLECULAR WEIGHT :: 380.40

WISWESSER LINE NOTATION :: QV1N1VQ2O2O2N1VQ1VQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 3587 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 16,807,1970

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 150 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information. Volume(issue)/page/year: AD691-490 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 84753 No. of Facilities: 111 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 664 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 84753 No. of Facilities: 103 (estimated) No. of Industries: 1 No. of Occupations: 4 No. of Employees: 1556 (estimated) No. of Female Employees: 1043 (estimated)

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
2

[ RTECS ]:
AH3760000

[ HS Code ]:
29225000

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 29225000

Articles

Functional consequence of the MET-T1010I polymorphism in breast cancer.

Oncotarget 6(5) , 2604-14, (2015)

Major breast cancer predisposition genes, only account for approximately 30% of high-risk breast cancer families and only explain 15% of breast cancer familial relative risk. The HGF growth factor rec...

Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis.

PLoS ONE 9(12) , e116152, (2014)

Pseudomonas syringae employs a type III secretion system to inject 20-30 different type III effector (T3SE) proteins into plant host cells. A major role of T3SEs is to suppress plant immune responses ...

Targeting glucose uptake with siRNA-based nanomedicine for cancer therapy.

Biomaterials 51 , 1-11, (2015)

Targeting cancer metabolism is emerging as a successful strategy for cancer therapy. However, most of the marketed anti-metabolism drugs in cancer therapy do not distinguish normal cells from cancer c...