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Z-Gly-Pro-AMC

Names

[ CAS No. ]:
68542-93-8

[ Name ]:
Z-Gly-Pro-AMC

[Synonym ]:
Z-GP-AMC
Z-Gly-Pro-AMC
Z-Gly-propmecounh
N-Carbobenzoxyglycylprolyl-4-methylcoumarinyl amide
Z-Gly-pro-NH-Mec
MFCD00038082

Biological Activity

[Description]:

Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1].

[Related Catalog]:

Research Areas >> Others
Signaling Pathways >> Others >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Z-Gly-Pro-AMC assay[1]: 1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 ℃. 2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM. 3. Fluorescence is measured kinetically for 30 min at 37 ℃.

[References]

[1]. Bracke A, et, al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160.

Chemical & Physical Properties

[ Density]:
1.358g/cm3

[ Boiling Point ]:
770.8ºC at 760 mmHg

[ Melting Point ]:
96-102ºC(lit.)

[ Molecular Formula ]:
C25H25N3O6

[ Molecular Weight ]:
463.48300

[ Flash Point ]:
420ºC

[ Exact Mass ]:
463.17400

[ PSA ]:
117.95000

[ LogP ]:
3.35910

[ Index of Refraction ]:
1.635

[ Storage condition ]:
−20°C

Safety Information

[ Safety Phrases ]:
S22;S24/25

[ WGK Germany ]:
3

Related Compounds