Stains-All

Names

[ Name ]:
Stains-All

[Synonym ]:
3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACORBOCYANINE
EINECS 231-047-7
DBTC
3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACARBOCYANINE
STAINS-ALL
4,5,4',5'-dibenzo-3,3'-diethyl-9-methylthiocarbocyanine bromide
MFCD00078788
Dibenzo-3,3'-diethyl-9-methylthiacarbocyanine-bromide
CARBOCYANIN DBTC

Biological Activity

[Description]:

Stains-All is a cationic carbocyanine dye.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Others

[In Vitro]

Almost all of the proteins found in skeletal muscle extracts, including the Ca2++Mg2+-ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca2+-binding proteins blue, Stains-all is a more sensitive stain for these Ca2+-binding proteins than is Coomassie blue[1].

[Kinase Assay]

Slab gels are fixed overnight with 25% isopropyl alcohol and washed exhaustively in 25% isopropyl alcohol to remove SDS. The gels are then stained in the dark for at least 48 h with 0.0025% Stains-all, 25% isopropyl alcohol, 7.5% formamide, and 30 mM Tris base, pH 8.8. The interaction of Stains-all with various Ca2+-binding proteins is also studied in aqueous solution. The standard solution contains 10 mM Tris base, pH 8.8, 0.001% Stains-all, and 0.1% formamide. Ca2+-binding proteins (0.5 to 12 μg) are added to 1.0 mL of solution and then incubated at room temperature in the dark for 30 min. The absorbance at 600 nm is then measured against a control solution, containing no protein, using a spectrophotometer[1].

[References]

[1]. Campbell KP, et al. Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all". J Biol Chem. 1983 Sep 25;258(18):11267-73.

[Related Small Molecules]

Chemical & Physical Properties

[ Molecular Formula ]:
C₃₀H₂₇BrN₂S₂

[ Molecular Weight ]:
559.58300

[ Exact Mass ]:
558.08000

[ PSA ]:
65.29000

[ LogP ]:
4.72030

[ Storage condition ]:
2-8°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Synthetic Route

Click to get detail synthetic route

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