Nudol

Nudol is a phenanthrene compound that has anti-cancer activity. Nudol inhibits cell proliferation, induces cell apoptosis. Nudol inhibits MMP-2M and MMP-9 activity (Ki: 988.9 nM, 1.76 μM, respectively). Nudol can be used in the research of cancers, such as osteosarcoma[1][2].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Metabolic Enzyme/Protease >> MMP

MMP-2:988.9 nM (Ki)

MMP-9:1.76 μM (Ki)

Nudol (0-40 μM, 24-72 h) decreases cell viability in several cancer cell lines[1]. Nudol (0-20 μM, 24/48 h) suppresses the cell migration and causes cell cycle arrest at G2/M phase in U2OS cells[1]. Nudol (20 μM, 48 h) induces cell apoptosis through the caspase-dependent pathway in U2OS cells[1]. Nudol (compound 4) inhibits MMP-2 and MMP-9 activity with Ki values of 988.9 nM and 1.76 μM respectively[2]. Cell Viability Assay[1] Cell Line: U2OS, MG63, MDA-MB-231, MCF-7, and A549 cells Concentration: 0, 10, 20, 30, 40 μM Incubation Time: 24, 48, and 72 h Result: Decreased the cell viability of osteosarcoma cells in dose- and time-dependent manners. Cell Cycle Analysis[1] Cell Line: U2OS cells Concentration: 0, 5, 10, 20 μM Incubation Time: 24, 48, and 72 h Result: Triggered G2/M phase arrest by decreasing cell cycle-related proteins (CDK1, CDK2, CDK4, and CDK10). Western Blot Analysis[1] Cell Line: U2OS cells Concentration: 0, 5, 10, 20, 40 μM Incubation Time: 24, 48, and 72 h Result: Increased the protein level of cytochrome c. Down-regulated anti-apoptotic Bcl-2, accompanied by an increased level of pro-apoptotic Bax.

[1]. Yuying Zhang, et al. Nudol, a phenanthrene derivative from Dendrobium nobile, induces cell cycle arrest and apoptosis and inhibits migration in osteosarcoma cells. Drug Des Devel Ther. 2019 Jul 29;13:2591-2601.

[2]. Mohammad Al-Amin, et al. Inhibitory Effect of Dioscorea bulbifera Tubers on the Migration of Triple-Negative Breast Cancer Cells. Breast Cancer Cells. Rev. Bras. Farmacogn. 31, 335–341 (2021).