23-(dipyrrometheneboron difluoride)-24-norcholesterol

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm)[1].

Research Areas >> Others

Signaling Pathways >> Others >> Others

BODIPY-cholesterol assay To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM[1]. Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration[1]. Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 µM), mixed thoroughly and incubated for 10 -20 mins at 37 °C[1]. The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment[1].

[1]. Daniel Wüstner, et al.Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28.

[2]. Maarit Hölttä-Vuori, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organiss. Traffic. 2008 Nov;9(11):1839-49.