Bz-Phe-Val-Arg-AMC hydrochloride salt

Names

[ Name ]:
Bz-Phe-Val-Arg-AMC hydrochloride salt

[Synonym ]:
bz-phe-val-arg-amc hcl
n-benzoyl-l-phe-val-arg-7-amido-4-methyl-coumarin hcl
benzoyl-phe-val-arg-7-amido-4-methylcoumarin hcl
n-benzoyl-phe-val-arg 7-amido-4-methylcoumarin
bz-phe-val-arg-amc
benzoyl-phe-val-arg-amc
benzoyl-fvr-amc
Thrombin Substrate III

Biological Activity

[Description]:

Bz-FVR-AMC is a fluorogenic substrate for procathepsin with a kcat/Km value of 1070 mM-1s-1. The high concentration of BZ-FVR-AMC inhibits the substrate[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Processing and activation of procathepsin S[2]: 1. Autocatalytic activation of procathepsin S was studied by incubation (final concentration 1-5 μM) at 37 ℃ in 0.5 mL of the appropriate buffer containing 2.5 mM dithiothreitol. 2. Aliquots of 10 μl were taken from the reaction mixture at the times indicated and mixed with 2.5 ml of substrate solution (10 μM Bz-FVR-AMC in 0.1 M phosphate buffer, pH 6.5, containing 1 mM EDTA (HY-Y0682) and 0.1% (w/v) polyethylene glycol 6000). 3. Fluorescence of the released AMC was then monitored continuously for 1 min in a pectrofluorimeter at excitation and emission wavelengths of 370 and 460 nm.

[References]

[1]. Vasiljeva O, et al. Recombinant human procathepsin S is capable of autocatalytic processing at neutral pH in the presence of glycosaminoglycans. FEBS Lett. 2005 Feb 14;579(5):1285-90.

[2]. Vasiljeva O, et al. Recombinant human cathepsin H lacking the mini chain is an endopeptidase. Biochemistry. 2003 Nov 25;42(46):13522-8.

Chemical & Physical Properties

[ Molecular Formula ]:
C₃₇H₄₃N₇O₆

[ Molecular Weight ]:
681.78100

[ Exact Mass ]:
681.32700

[ PSA ]:
208.51000

[ LogP ]:
5.40670

MSDS

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Safety Information

[ WGK Germany ]:
3