Biotin-LC-LC-NHS

Names

[ Name ]:
Biotin-LC-LC-NHS

[Synonym ]:
(2,5-dioxopyrrolidin-1-yl) 6-[6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoylamino]hexanoate
1H-Thieno[3,4-d]imidazole-4-pentanamide, N-[6-[[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]amino]-6-oxohexyl]hexahydro-2-oxo-, (3aS,4S,6aR)-
MFCD00467154
N-{6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl}-6-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanamide

Biological Activity

[Description]:

Biotin-LC-LC-NHS is a SMCC cross-linking reagent that can be used to mark antibody and other small molecules, such as Paclitaxel[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Biotinylation of antibodies is accomplished by the reaction of Biotin-LC-LC-NHS (1 mg/mL in dimethyl formamide) with the protein in aqueous phase pH 7.4. The molar ratio of biotin to antibody in the labeling reaction was 10:1 (the final concentration of biotin was 70 μM) [1].

[References]

[1]. K Dill, et al. Immunoassays Based on Electrochemical Detection Using Microelectrode Arrays. Biosens Bioelectron. 2004 Nov 1;20(4):736-42.

[2]. Lev G Lis, et al. Synthesis and Biological Evaluation of a Biotinylated Paclitaxel With an Extra-Long Chain Spacer Arm. ACS Med Chem Lett . 2012 Sep 13;3(9):745-748.

Chemical & Physical Properties

[ Density]:
1.3±0.1 g/cm3

[ Melting Point ]:
147-151ºC

[ Molecular Formula ]:
C₂₆H₄₁N₅O₇S

[ Molecular Weight ]:
567.698

[ Exact Mass ]:
567.272644

[ PSA ]:
188.31000

[ LogP ]:
-1.83

[ Index of Refraction ]:
1.579

Safety Information

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Articles

Preparation of modified tubulins.

Meth. Enzymol. 196 , 478-485, (1991)

Ligand-based histochemical localization and capture of cells expressing heat-stable enterotoxin receptors.

Mol. Microbiol. 8 , 865-873, (1993)

The heat stable enterotoxins (ST) of enterotoxigenic Escherichia coli (ETEC) cause diarrhoea by binding specific intestinal receptors. Precise histochemical localization of ST receptors could provide ...