Trypsin

[Description]:

Trypsin is an enzyme that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6].

[Related Catalog]:

Research Areas >> Infection

Signaling Pathways >> GPCR/G Protein >> Protease-Activated Receptor (PAR)

Research Areas >> Inflammation/Immunology

[Target]

PAR2, PAR4[6]

[In Vitro]

Trypsin (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2]. Trypsin (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2]. Trypsin (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3]. Trypsin (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4]. Western Blot Analysis[2] Cell Line: LLC-PK cells, ST cells Concentration: 5 μg/mL Incubation Time: 24 or 48 h Result: Promoted PDCoV replication in LLC-PK cells but not ST cells. Immunofluorescence[2] Cell Line: LLC-PK cells, ST cells Concentration: 10 and 50 ng/mL Incubation Time: 12 h Result: Significantly increased cell-to-cell fusion activity during PDCoV infection of LLC-PK cells.

[In Vivo]

Trypsin (100-500 μg per site in 50 μL saline, intradermal injection) induces scratching behaviour in mice[5]. Animal Model: Swiss mice[5] Dosage: 100-500 μg per site, in saline (50 μL) Administration: Intradermal injection Result: Induced pruritus, and was inhibited by trypsin inhibitor.

[References]

[1]. Bhupendra S.Kaphalia. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289.

[2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468.

[3]. H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8.

[4]. Michael J. V. White, et al. Trypsin Potentiates Human Fibrocyte Differentiation. PLoS One. 2013; 8(8): e70795.

[5]. R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103.

[6]. F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82.

[ Melting Point ]:
115°C

[ Molecular Formula ]:
C₆H₁₅O₁₂P₃

[ Molecular Weight ]:
372.1

[ Appearance of Characters ]:
lyophilized powder

[ Storage condition ]:
−20°C

[ Stability ]:
Stable. Incompatible with strong oxidizing agents.

[ Water Solubility ]:
Reconstitute in aqueous buffer | Soluble in water (10 mg/ml), phosphate buffers (10 mg/ml), and balanced salt solutions (1 mg/ml).

[ Symbol ]:

GHS07, GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H315-H319-H334-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338-P342 + P311

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Faceshields;Gloves

[ Hazard Codes ]:
Xn,B

[ Risk Phrases ]:
36/37/38-42-42/43

[ Safety Phrases ]:
22-24-26-36/37-45-23

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
2

[ RTECS ]:
GC3050000

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