ZIKV-IN-3

ZIKV-IN-3 (compound 5a), an andrographolide derivatives, is a potent ZIKV NS5 methyl transferase (MTase) inhibitor with an IC50 value of 18.34 μM. ZIKV-IN-3 inhibits ZIKV replication and infection. ZIKV-IN-3 can be used in research of Zika virus (ZIKV)[1].

Research Areas >> Infection

Signaling Pathways >> Epigenetics >> DNA Methyltransferase

IC50: 18.34 μM (MTase)[1]

ZIKV-IN-2 (compound 5a; 0.08-50 μM) inhibits ZIKV-induced plaque formation with an EC50 value of 0.76 μM and has anti-ZIKV effect with EC50 values of 0.48, 0.37, and 0.45 μM for Vero, Huh7 and A549 cells, respectively[1]. ZIKV-IN-2 (0.08-50 μM; 48 h) inhibits the expression of ZIKV E protein with an EC50 value of 0.38 μM and inhibits ZIKV replication and infection[1]. ZIKV-IN-2 (0.08-50 μM; 96 h) has low cytotoxicity in Vero, Huh7 and A549 cells[1].Vero, Huh7 and A549 cells[1]. Cell Viability Assay[1] Cell Line: Vero, Huh7 and A549 cells Concentration: 0.08, 0.4, 2, 10, and 50 μM Incubation Time: 96 hours Result: Exhibited low cytotoxicities to Vero cells, Huh7 cells and A549 cells with CC50>200 μM to all three cell lines. Western Blot Analysis[1] Cell Line: Vero cells Concentration: 0.08, 0.4, 2, 10, and 50 μM Incubation Time: 48 hours Result: Inhibited the expression of ZIKV E protein in a dose-dependent manner.

[1]. Qian W, et al. Discovery of dehydroandrographolide derivatives with C19 hindered ether as potent anti-ZIKV agents with inhibitory activities to MTase of ZIKV NS5. Eur J Med Chem. 2022 Aug 27;243:114710.