1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-iodopyrimidine-2,4-dione,hydrate

Idoxuridine (5-Iodo-2′-deoxyuridine, 5-IUdR, IdUrd) hydrate is an iodinated thymidine analogue that competitively inhibits phosphorylases. Idoxuridine can inhibit viral activity, particularly viral eye infections, including herpes simplex keratitis, by inhibiting DNA polymerase and affecting viral replication. Idoxuridine against feline herpesvirus has the IC50 value of 4.3 μM[1].

Signaling Pathways >> Metabolic Enzyme/Protease >> Phosphatase

Research Areas >> Infection

Idoxuridine (2-10 μM, 72 hours) hydrate has the IC50 value of 4.3 μM of antiviral[1]. Cell Proliferation Assay[1] Cell Line: Crandell-Reese feline kidney (CRFK) cells Concentration: 2-10 μM Incubation Time: 72 hours Result: Showed the IC50 value of 4.3 μM. Cell Cytotoxicity Assay[1] Cell Line: Crandell-Reese feline kidney (CRFK) cells Concentration: 5-50 μM Incubation Time: 72 hours Result: Reduced by 10.8% relatively in CRFK cells.

Idoxuridine (intraperitoneal injection, 50-200 mg/kg, 3 times, 3 hours interval) hydrate can stimulate the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells (SRBC) in C3HeB/FeJ female and male mice and A/J male mice[2]. Animal Model: C3HeB/FeJ female and male mice and A/J male mice, aged 2 to 4 months[2] Dosage: 50-200 mg/kg Administration: Intraperitoneal injection, 3 times, 3 hours interval Result: Stimulated the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells (SRBC) in the dose range of 50-200 mg/kg.

[1]. ]David J Maggs, et al. In vitro efficacy of ganciclovir, cidofovir, penciclovir, foscarnet, idoxuridine, and acyclovir against feline herpesvirus type-1. Am J Vet Res. 2004 Apr;65(4):399-403.

[2]. D E Griswold, et al. Stimulation of hemolysin plaque-forming cells by idoxuridine. Cancer Res. 1975 Jan;35(1):88-92.