MMP-9-IN-3

MMP-9-IN-3 is a MMP-9 inhibitor (IC50: 5.56 nM) that forms hydrogen bond with MMP-9. MMP-9-IN-3 also inhibits AKT activity (IC50: 2.11 nM). MMP-9-IN-3 shows cell cytotoxicity and induces cell apoptosis. MMP-9-IN-3 can be used in the research of cancers[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> PI3K/Akt/mTOR >> Akt

Signaling Pathways >> Metabolic Enzyme/Protease >> MMP

MMP-9:5.56 nM (IC50)

MMP-1:447 nM (IC50)

MMP-2:221 nM (IC50)

MMP13:295 nM (IC50)

AKT:2.11 nM (IC50)

MMP-9-IN-3 (compound 28, 0-1 μM approximately, 72 h) shows cytotoxicity to Wi-38, MCF-7, NFS-60, HepG-2 cells[1]. MMP-9-IN-3 (72 h) induces apoptosis in MCF-7, NFS-60 and HepG-2 cells at 6.6 nM, 5.8 nM, 5.7 nM respectively[1]. MMP-9-IN-3 (72 h) induces significant caspase 3/7 activation in MCF-7, NFS-60 and HepG-2 cells at 6.6 nM, 5.8 nM, 5.7 nM respectively[1]. MMP-9-IN-3 (5.7 nM, 24 h) inhibits cell migration in HepG-2 cells[1]. Cell Viability Assay[1] Cell Line: Wi-38, MCF-7, NFS-60, HepG-2 cells Concentration: 0-1 μM approximately Incubation Time: 72 h Result: Showed cytotoxicity with IC50: 314 nM (Wi-38), 6.6 nM (MCF-7), 5.8 nM (NFS-60), 5.7 nM (HepG-2). Cell Migration Assay [1] Cell Line: HepG-2 Concentration: 5.7 nM Incubation Time: 24 h Result: Inhibited cell migration by 71.25%.

[1]. Mohammed Salah Ayoup, et al. Battle tactics against MMP-9; discovery of novel non-hydroxamate MMP-9 inhibitors endowed with PI3K/AKT signaling attenuation and caspase 3/7 activation via Ugi bis-amide synthesis. Eur J Med Chem. 2020 Jan 15;186:111875.