ZnAF-1F

Names

[ Name ]:
ZnAF-1F

[Synonym ]:
ZnAF-1F
5-{2-[Bis(2-pyridylmethyl)amino]ethylamino}-2',7'-difluorofluorescein

Biological Activity

[Description]:

ZnAF-1F is a potent fluorophore for with an Kd value of 2.2 nM. ZnAF-1F can be used as fluorescent probes for Zn2+ in cells. ZnAF-1F shows λ excitation of 489 nm and λ emission of 514 nm[1][2][3].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

ZnAF-1F (1 µM) shows an Kon value of 3.5*106 M-1s-1, and an Koff value of 7.7*10-3 s-1 in 100mM HEPES buffer[1]. ZnAF-1F is a Zn sensors in neutral and slightly acidic conditions[2].

[References]

[1]. Hirano T, et al. Improvement and biological applications of fluorescent probes for zinc, ZnAFs. J Am Chem Soc. 2002 Jun 12;124(23):6555-62.

[2]. Zhaohua Dai, et al. Tailoring tripodal ligands for zinc sensing. New J. Chem., 2007,31, 1708-1718.

[3]. Que EL, et al. Metals in neurobiology: probing their chemistry and biology with molecular imaging. Chem Rev. 2008 May;108(5):1517-49.

Chemical & Physical Properties

[ Density]:
1.49g/cm3

[ Boiling Point ]:
822.1ºC at 760 mmHg

[ Molecular Formula ]:
C₃₄H₂₆F₂N₄O₅

[ Molecular Weight ]:
608.59100

[ Flash Point ]:
451ºC

[ Exact Mass ]:
608.18700

[ PSA ]:
128.79000

[ LogP ]:
6.22410

[ Index of Refraction ]:
1.715

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

Articles

Improvement and biological applications of fluorescent probes for zinc, ZnAFs.

J. Am. Chem. Soc. 124 , 6555-6562, (2002)

The development and cellular applications of novel fluorescent probes for Zn2+, ZnAF-1F, and ZnAF-2F are described. Fluorescein is used as a fluorophore of ZnAFs, because its excitation and emission w...