Rhodamine 123

Names

[ Name ]:
Rhodamine 123

[Synonym ]:
RHODAMINE METHYL ESTER CHLORIDE
Xanthylium, 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]-, chloride (1:1)
2-(6-amino-3-imino-3H-xanthen-9-yl)-methyl ester,hydrochloride
rh123
3,6-Diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride
MFCD00012664
RHODAMINE 123 HYDROCHLORIDE
Rhodamine 123
RHODAMINE 123,PURE
XANTHYLIUM, 3,6-DIAMINO-9-(2-(METHOXYCARBONYL)PHENYL)-, CHLORIDE
rhodamine 110 methyl ester
Rhodamine-123
EINECS 263-687-8

Biological Activity

[Description]:

Rhodamine 123 is a fluorescent dye (λex=503 nm, λem=527 nm).

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Cardiovascular Disease

Dye Reagents

[In Vitro]

The intensity of R123 fluorescence has a peak at concentration of 50 μM, and decreases to zero at higher concentrations due to self-quenching[1].

[Kinase Assay]

Measurements are made at room temperature with continuous stirring of the mitochondrial suspension using spectrophotometer equipped with a magnetic stirrer with fluorescent cation R123 as probe. Excitation and emission wavelengths are 503 nm and 527 nm, respectively. The incubation medium is the respiration buffer. R123 and sodium pyruvate are added to final concentrations of 50 nM and 10 mM, respectively. Isolated mitochondria maintain a steady membrane potential (±5%) throughout the duration of the recording[1].

[References]

[1]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285.

[Related Small Molecules]

Chemical & Physical Properties

[ Melting Point ]:
235 °C

[ Molecular Formula ]:
C₂₁H₁₇ClN₂O₃

[ Molecular Weight ]:
380.824

[ Exact Mass ]:
380.092773

[ PSA ]:
89.31000

[ LogP ]:
5.53580

[ Water Solubility ]:
Partially soluble

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: ZE0883000

CHEMICAL NAME :: Xanthylium, 3,6-diamino-9-(2-(methoxycarbonyl)phenyl)-, chloride

CAS REGISTRY NUMBER :: 62669-70-9

LAST UPDATED :: 199701

DATA ITEMS CITED :: 3

MOLECULAR FORMULA :: C21-H17-N2-O3.Cl

MOLECULAR WEIGHT :: 380.85

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 60 mg/kg

SEX/DURATION :: female 7-10 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - other effects to embryo

REFERENCE :: TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1980- Volume(issue)/page/year: 9,29,1989

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 60 mg/kg

SEX/DURATION :: female 7-10 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants) Reproductive - Specific Developmental Abnormalities - musculoskeletal system

REFERENCE :: TJADAB Teratology, The International Journal of Abnormal Development. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968- Volume(issue)/page/year: 40,143,1989

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xn: Harmful;

[ Risk Phrases ]:
R22

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
ZE0883000

[ HS Code ]:
2932999099

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2932999099

[ Summary ]:
2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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