Cyanine5 NHS ester bromide

Cyanine5 NHS ester bromide is a active compound, can be used to label amino groups in peptides, proteins, and oligonucleotides. Cyanine5 NHS ester bromide is a cyanine dye, fluorescence-labeling neurotensin (8-13) via arginine residues[1].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Cyanine5 NHS ester bromide can be excitable with a red (635 nm) and a 488 nm argon laser, respectively[1]. Cyanine5 NHS ester bromide applys for characterization[1]: 1. Acquire high resolution mass spectra (HRMS) with Agilent 6540 UHD Accurate-Mass QTOF LC/MS system coupled to an Agilent 1290 HPLC system, using an ESI source. 2. Perform preparative HPLC with a system from Knauer (Berlin, Germany). A Kinetex-XB C18, 5 μm, 250 × 21 mm (Phenomenex, Aschaffenburg, Germany) is used as stationary phase. Mixtures of 0.1% aq TFA and acetonitrile are used as mobile phase. The flow rate is 18-20 mL/min, and a detection wavelength of 220 nm is used throughout. 3. Analysis HPLC with a system from Agilent Technologies. A Kinetex-XB C18, 2.6 μm, 100 × 3 mm (Phenomenex) serves as stationary phase at a flow rate of 0.5-0.6 mL/min. The oven temperature is set to 25 °C. Mixtures of 0.04% aq TFA and acetonitrile are used as mobile phase. The injection volume is 20 μL. Detection is performed at 220 nm.

[1]. Keller M, et al. Fluorescence Labeling of Neurotensin(8-13) via Arginine Residues Gives Molecular Tools with High Receptor Affinity. ACS Med Chem Lett. 2019 Nov 19;11(1):16-22.