Apoptosis inducer 9

Apoptosis inducer 9 induces apoptosis with IC50 value of 4.21 μM. Apoptosis inducer 9 induces apoptosis through the mitochondrial pathway and enhance the expression of Cl-caspase-3, Cl-caspase-9 and Cl-PARP. Apoptosis inducer 9 can be used the potential to develop new anti-proliferative agents[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Apoptosis inducer 9 (Compound A1) (5-10 μM, 12 hours) induces apoptosis in HepG-2 cells in a concentration-dependent manner[1]. Apoptosis inducer 9 (Compound A1) (0-20 μM, 24 hours) triggers Cl-caspase-3, Cl-caspase-9 and Cl-PARP protein expression in HepG-2, thereby inducing apoptosis[1]. Apoptosis inducer 9 (Compound A1) (0-20 μM, 24 hours) induces apoptosis by triggering the mitochondrial apoptosis pathway[1]. Cell Viability Assay[1] Cell Line: HepG-2 cells Concentration: 5 and 10 μM Incubation Time: 12 hours Result: Apoptotic bodies appeared shrunken, nuclei shrunken and cells fragmented. The apoptosis rates at concentrations of 5 μM and 10 μM were 10.2% and 42.7%, respectively. Western Blot Analysis[1] Cell Line: HepG-2 cells Concentration: 0, 5, 10 and 20 μM Incubation Time: 24 hours Result: Triggered the caspase cascade in HepG-2 and the corresponding protein expression gradually increased. Western Blot Analysis[1] Cell Line: HepG-2 cells Concentration: 0, 5, 10 and 20 μM Incubation Time: 24 hours Result: Induced up-regulation of the expression of Bcl-2, Bax and p53 proteins, and increased the ratio of Bax/Bcl-2.

[1]. Xiao S, et al. Novel panaxadiol triazole derivatives induce apoptosis in HepG-2 cells through the mitochondrial pathway. Bioorg Chem. 2020 Sep;102:104078.