MMP-9-IN-5

MMP-9-IN-5 is a MMP-9 inhibitor (IC50: 4.49 nM) that forms hydrogen bond with MMP-9. MMP-9-IN-5 also inhibits AKT activity (IC50: 1.34 nM). MMP-9-IN-5 shows cell cytotoxicity and induces cell apoptosis. MMP-9-IN-5 can be used in the research of cancers[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> PI3K/Akt/mTOR >> Akt

Signaling Pathways >> Metabolic Enzyme/Protease >> MMP

MMP-9:4.49 nM (IC50)

MMP-1:104 nM (IC50)

MMP-2:266 nM (IC50)

MMP-13:280 nM (IC50)

AKT:1.34 nM (IC50)

MMP-9-IN-5 (compound 8, 0-1 μM approximately, 72 h) shows cytotoxicity to Wi-38, MCF-7, NFS-60, HepG-2 cells[1]. MMP-9-IN-5 (72 h) induces apoptosis in MCF-7, NFS-60 and HepG-2 cells at 6.9 nM, 5.5 nM, 3.1 nM respectively[1]. MMP-9-IN-5 (72 h) induces significant caspase 3/7 activation in MCF-7, NFS-60 and HepG-2 cells at 6.9 nM, 5.5 nM, 3.1 nM respectively [1]. MMP-9-IN-5 (3.1 nM, 24 h) inhibits cell migration in HepG-2 cells[1]. Cell Viability Assay[1] Cell Line: Wi-38, MCF-7, NFS-60, HepG-2 cells Concentration: 0-1 μM approximately Incubation Time: 72 h Result: Showed cytotoxicity with IC50s: 35.1 nM (Wi-38), 6.9 nM (MCF-7), 5.5 nM (NFS-60), 3.1 nM (HepG-2). Cell Migration Assay [1] Cell Line: HepG-2 Concentration: 3.1 nM Incubation Time: 24 h Result: Inhibited cell migration by 59.35%.

[1]. Mohammed Salah Ayoup, et al. Battle tactics against MMP-9; discovery of novel non-hydroxamate MMP-9 inhibitors endowed with PI3K/AKT signaling attenuation and caspase 3/7 activation via Ugi bis-amide synthesis. Eur J Med Chem. 2020 Jan 15;186:111875.