- Shanghai Nianxing Industrial Co., Ltd
- China
- Product Name: LF3
- Price:
- Purity: 98.0%
- Stocking Period: 10 Day
- Contact: Huang
- ShangHai DC Chemicals Co.,Ltd
- China
- Product Name: LF3
- Price: ￥Inquiry/1g
- Purity: 98.9%
- Stocking Period: 1 Day
- Contact: Tony Lai
- DC Chemicals Limited
- China
- Product Name: LF3
- Price: $400.0/100mg $750.0/250mg $1500.0/1g
- Purity: 98.0%
- Stocking Period: 3 Day
- Contact: Tony Cao
664969-54-4
664969-54-4 structure
- Name: LF3
- Chemical Name: LF3
- CAS Number: 664969-54-4
- Molecular Formula: C20H24N4O2S2
- Molecular Weight: 416.560
- Catalog: Signaling Pathways Stem Cell/Wnt β-catenin
- Create Date: 2018-05-20 19:48:17
- Modify Date: 2024-01-06 21:57:00
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LF3 is an antagonist of the β-Catenin/TCF4 interaction with antitumor activity; has an IC50 of 1.65 μM.
| Name | LF3 |
|---|---|
| Synonyms |
1-Piperazinecarbothioamide, N-[4-(aminosulfonyl)phenyl]-4-[(2E)-3-phenyl-2-propen-1-yl]-
4-[(2E)-3-Phenyl-2-propen-1-yl]-N-(4-sulfamoylphenyl)-1-piperazinecarbothioamide N-[4-(aminosulfonyl)phenyl]-4-(3-phenylprop-2-enyl)piperazine-1-carbothioamide |
| Description | LF3 is an antagonist of the β-Catenin/TCF4 interaction with antitumor activity; has an IC50 of 1.65 μM. |
|---|---|
| Related Catalog | |
| Target |
IC50: 1.65 μM (β-Catenin/TCF4, AlphaScreen), 1.82 μM (β-Catenin/TCF4, ELISA)[1] |
| In Vitro | LF3 inhibits Wnt/β-catenin signals in cells with exogenous reporters and in colon cancer cells with endogenously high Wnt activity. LF3 also suppresses features of cancer cells related to Wnt signaling, including high cell motility, cell-cycle progression, and the overexpression of Wnt target genes. However, LF3 does not cause cell death or interfere with cadherin-mediated cell-cell adhesion. Remarkably, the self-renewal capacity of cancer stem cells is blocked by LF3 in concentration-dependent manners[1]. |
| In Vivo | LF3 reduces tumor growth and induces differentiation in a mouse xenograft model of colon cancer. Tumor growth is significantly reduced when mice with GFPhigh cells are treated with LF3 at 50 mg/kg. LF3 treatment does not disturb the normal histology of the gut of mice[1]. |
| Cell Assay | LF3 is dissolved in DMSO to a concentration of 50 mM and diluted with culture medium. Two colon cancer cell lines (HCT116 and HT29) and a breast cancer cell line (MCF7) are treated with LF3 (0, 30, 60 μM) for 24 hours and labeled with BrdUrd for 4 to 5 hours to detect proliferating cells[1]. |
| Animal Admin | Mice: Unsorted GFPlow and GFPhigh SW480 cells are subcutaneously injected into the back skin of NOD/SCID mice. Tumor growth is monitored over a period of 45 days. For therapy, LF3 is administered i.v. at 50 mg/kg body weight for three rounds over 5 consecutive days, with 2-day breaks[1]. |
| References |
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Boiling Point | 609.6±65.0 °C at 760 mmHg |
| Molecular Formula | C20H24N4O2S2 |
| Molecular Weight | 416.560 |
| Flash Point | 322.5±34.3 °C |
| Exact Mass | 416.134064 |
| LogP | 1.97 |
| Vapour Pressure | 0.0±1.7 mmHg at 25°C |
| Index of Refraction | 1.680 |
| Storage condition | -20℃ |