Shanghai Nianxing Industrial Co., Ltd
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Product Name: H100?
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643727-55-3

643727-55-3 structure

643727-55-3 structure

Name: H100
Chemical Name: H100
CAS Number: 643727-55-3
Molecular Formula: C₁₈H₁₆N₂O₆S
Molecular Weight: 388.39
Catalog: Signaling Pathways Membrane Transporter/Ion Channel Chloride Channel
Create Date: 2018-04-27 08:28:32
Modify Date: 2024-01-23 17:59:36
H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.

Name	H100

Description	H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.
Related Catalog	Signaling Pathways >> Membrane Transporter/Ion Channel >> Chloride Channel Research Areas >> Others
Target	Cl- transport[1]
In Vitro	H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter (NKCC) and the Band 3 anion exchanger (AE), but no effect against KCl cotransporter (KCC), in human erythrocytes. H100 (0.1 mM) shows 63% and 74% inhibition of NKCC and AE in human erythrocytes[1].
Cell Assay	35SO42- efflux is used as a convenient measure of the activity of the Band 3 anion exchanger (AE). Erythrocytes are suspended into MBS containing Na2SO4 rather than NaCl and incubated at 37°C for 30 min. The cell suspension is then centrifuged (3,000 g, 5 min) and the supernatant removed. The above procedure is repeated twice to ensure the intracellular replacement of Cl- with SO42-. To load the erythrocytes with radiolabel, the packed cells are re-suspended to approximately 50% haematocrit in a solution containing 1 part SO42- MBS and 9 parts of a medium containing (in mM) 300 sucrose and 10 MOPS (pH 7.4, 300 ± 5 mOsm) and placed in a microcentrifuge tube. 10 μCi of 35SO42- is then added and the suspension incubated at 37°C for 1 h. At the end of this period, the cells are then washed four times by centrifugation (10,000 g, 10 s) in ice-cold SO42- MBS. All inhibitors (H100) are dissolved in either DMSO or MBS and are added to the cell suspensions prior to the addition of the radioisotope[1].
References	[1]. Culliford S1, et al. Specificity of classical and putative Cl(-) transport inhibitors on membrane transport pathways in human erythrocytes. Cell Physiol Biochem. 2003;13(4):181-8.

Molecular Formula	C₁₈H₁₆N₂O₆S
Molecular Weight	388.39
Storage condition	2-8℃