909089-13-0
909089-13-0 structure
- Name: SKL2001
- Chemical Name: 5-(2-Furyl)-N-[3-(1H-imidazol-1-yl)propyl]-1,2-oxazole-3-carboxamide
- CAS Number: 909089-13-0
- Molecular Formula: C14H14N4O3
- Molecular Weight: 286.286
- Catalog: Signaling Pathways Stem Cell/Wnt Wnt
- Create Date: 2018-07-19 22:01:18
- Modify Date: 2024-01-05 12:30:54
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SKL2001 is an agonist of the Wnt/β-catenin pathway, with anti-cancer activity.
| Name | 5-(2-Furyl)-N-[3-(1H-imidazol-1-yl)propyl]-1,2-oxazole-3-carboxamide |
|---|---|
| Synonyms |
5-(2-Furyl)-N-[3-(1H-imidazol-1-yl)propyl]-1,2-oxazole-3-carboxamide
3-Isoxazolecarboxamide, 5-(2-furanyl)-N-[3-(1H-imidazol-1-yl)propyl]- SKL2001 |
| Description | SKL2001 is an agonist of the Wnt/β-catenin pathway, with anti-cancer activity. |
|---|---|
| Related Catalog | |
| Target |
Wnt/β-catenin[1] |
| In Vitro | SKL2001 is an agonist of the Wnt/β-catenin pathway, and also upregulates the expression of Axin2, a downstream target of the Wnt/β-catenin pathway, but shows no effect on NF-κB, p53 reporter activity and GSK-3β activity. SKL2001 causes osteoblast differentiation (20 and 40 μM) and suppresses preadipocyte differentiation (5, 10, and 30 μM) via the activation of the Wnt/β-catenin pathway. SKL2001 (5, 10, and 30 μM) stabilizes intracellular β-catenin in 3T3-L1 cells[1]. SKL2001 (40 μM) significantly inhibits the proliferation of HCT116 spheroids independently of cytotoxicity and the inhibition is reversible; SKL2001 cuases cell cycle arrest in HCT116 spheroids. SKL2001 (40 μM) enhances round-shape spheroid formation and E-cadherin expression[2]. |
| Cell Assay | ST2 cells are cultured on glass chamber slides and then treated with DMSO or SKL2001 for 15 h. After treatment, the cells are washed with PBS, fixed with 4% formaldehyde, permeabilized in 0.3% Triton X-100, and blocked in 4% bovine serum albumin for 1 h. The cells are stained with anti-β-catenin antibody and then analyzed by confocal microscopy using a microscope[1]. |
| References |
| Density | 1.4±0.1 g/cm3 |
|---|---|
| Boiling Point | 595.7±50.0 °C at 760 mmHg |
| Molecular Formula | C14H14N4O3 |
| Molecular Weight | 286.286 |
| Flash Point | 314.1±30.1 °C |
| Exact Mass | 286.106598 |
| LogP | 0.37 |
| Vapour Pressure | 0.0±1.7 mmHg at 25°C |
| Index of Refraction | 1.655 |
| Storage condition | -20℃ |