1522117-83-4

1522117-83-4 structure

Name: FBBBE
Chemical Name: 3',6'-Bis{[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)oxy]-3H-[spiro(isobenzofuran-1,9,-xanthen]-3-one} FBBBE
CAS Number: 1522117-83-4
Molecular Formula: C₄₆H₄₆B₂O₉
Molecular Weight: 584.229
Catalog: Research Areas Cancer
Create Date: 2018-07-21 03:47:41
Modify Date: 2024-01-30 07:06:38
FBBBE is used to detect the production of H2O2 by cells. FBBBE can be triggered by intracellular H2O2 and converted to fluorescein, resulting in an increase in intracellular fluorescence (Ex=4480 nm, Em=512 nm)[1].

Name	3',6'-Bis{[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)oxy]-3H-[spiro(isobenzofuran-1,9,-xanthen]-3-one} FBBBE
Synonyms	3',6'-BIS{[4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)OXY]-3H-[SPIRO(ISOBENZOFURAN-1,9,-XANTHEN]-3-ONE}FBBBE

Description	FBBBE is used to detect the production of H2O2 by cells. FBBBE can be triggered by intracellular H2O2 and converted to fluorescein, resulting in an increase in intracellular fluorescence (Ex=4480 nm, Em=512 nm)[1].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Others >> Others Research Areas >> Inflammation/Immunology
Target	H2O2[1]
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. The RAW 264.7 cells are seeded at 250,000 in glass bottom 35 mm dishes and post-adhering (5 h). 2. Some dishes are treated with 1 μg/mL of LPS for 24 h. Other dishes are treated the next day after plating with either 100 μM H2O2 for 1 h or PBS for 1 h. 3. Cells are washed twice with PBS and fixed in ice-cold 95% ethanol for 15 min. 3. Cells are twice rinsed with PBS and incubated with either 50 μM probe (FBBBE) for 1 h at room temperature. 4. Cells are washed twice with PBS and coverslips were mounted over cells using Aqua Mount. 5. Confocal images are obtained at 80× magnification on an Olympus FV1000 confocal laser scanning microscope. 6. Images were processed using ImageJ software.
References	[1]. Daniel, K.B., et al. Readily accessible fluorescent probes for sensitive biological imaging of hydrogen peroxide. Chembiochem 14(5), 593-598 (2013).