1802078-31-4

1802078-31-4 structure

Name: Mca-(Asn670,Leu671)-Amyloid β/A4 Protein Precursor770 (667-675)-Lys(Dnp) ammonium acetate salt
Chemical Name: Mca-(Asn670,Leu671)-Amyloid β/A4 Protein Precursor770 (667-675)-Lys(Dnp) ammonium acetate salt
CAS Number: 1802078-31-4
Molecular Formula: C₆₈H₈₈N₁₄O₂₇
Molecular Weight: 1533.50
Catalog: Signaling Pathways Neuronal Signaling Beta-secretase
Create Date: 2021-01-15 10:40:28
Modify Date: 2024-04-07 10:35:50
Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.

Name	Mca-(Asn670,Leu671)-Amyloid β/A4 Protein Precursor770 (667-675)-Lys(Dnp) ammonium acetate salt
Synonyms	L-Lysine, N-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-seryl-L-α-glutamyl-L-valyl-L-asparaginyl-L-leucyl-L-α-aspartyl-L-alanyl-L-α-glutamyl-L-phenylalanyl-N6-(2,4-dinitrophenyl)- N-[(7-Methoxy-2-oxo-2H-chromen-4-yl)acetyl]-L-seryl-L-α-glutamyl-L-valyl-L-asparaginyl-L-leucyl-L-α-aspartyl-L-alanyl-L-α-glutamyl-L-phenylalanyl-N6-(2,4-dinitrophenyl)-L-lysine

Description	Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
Related Catalog	Signaling Pathways >> Neuronal Signaling >> Beta-secretase Research Areas >> Others
References	[1]. Vila-Real H, et al. Peptidomimetic β-Secretase Inhibitors Comprising a Sequence of Amyloid-β Peptide for Alzheimer's Disease. J Med Chem. 2015 Jul 23;58(14):5408-18. [2]. Yen YC, et al. Development of an Efficient Enzyme Production and Structure-Based Discovery Platform for BACE1 Inhibitors. Biochemistry. 2019 Nov 5;58(44):4424-4435.