70375-24-5

70375-24-5 structure

Name: boc-val-pro-arg-amc hcl
Chemical Name: tert-butyl N-[1-[2-[[5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]carbamoyl]pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]carbamate,hydrochloride
CAS Number: 70375-24-5
Molecular Formula: C₃₁H₄₆ClN₇O₇
Molecular Weight: 664.19300
Catalog: Biochemical Amino acids and their derivatives Arginine derivatives
Create Date: 2018-11-25 09:29:36
Modify Date: 2024-01-08 21:47:26
Boc-Val-Pro-Arg-MCA hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity[1].

Name	tert-butyl N-[1-[2-[[5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]carbamoyl]pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]carbamate,hydrochloride
Synonyms	Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride MFCD00058181

Description	Boc-Val-Pro-Arg-MCA hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity[1].
Related Catalog	Research Areas >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 胰蛋白酶活性测定[2]1. 将稀释的 Trypsin 酶 (100 μL) 加入到 800 μL 的 50mM Tris-HCl 缓冲液 (pH 8.0) 中。 2. 加入 100 μL 50 μM 底物 Boc-Val-Pro-Arg-MCA 可立即引发反应。 3. 55°C 孵育 10 分钟。 4. 加入 1.5 mL 停止剂 (甲醇:正丁醇:蒸馏水= 35:30:35,v/v/v) 停止反应。 5. 用荧光光谱仪测量荧光强度 (excitation 380 nm, emission 450 nm)。
References	[1]. Liu JY, et al. Purification and characterization of a sarcoplasmic serine proteinase from threadfin bream Nemipterus virgatus muscle. Food Chem. 2019 Jun 30;284:198-204. [2]. Sriket C, et al. Low molecular weight trypsin from hepatopancreas of freshwater prawn (Macrobrachium rosenbergii): Characteristics and biochemical properties. Food Chemistry, 2012, 134(1): 351-358.

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport
WGK Germany	3