| In Vitro |
EGFR-IN-50 (0-10 μM; 72 h) selectively inhibits NSCLC cell line H3255 (expressing EGFR-L858R) compared to other tumor cells[1]. EGFR-IN-50 (1-10 μM; 72 h) induces cell cycle arrest in G0-G1 phase[1]. EGFR-IN-50 (0.039-10 μM; 4 h) inhibits the phosphorylation of EGFR in H3255 cells[1]. Cell Proliferation Assay[1] Cell Line: A549, H3255, HepG2, MCF-7, HT-29 and A431 cells Concentration: 0-10 μM Incubation Time: 72 hours Result: Showed GI50 values of >10, and 0.7873 μM for A549 and H3255 cells, respectively. Showed IC50 values of >10, 7.309, >10 and 6.703 μM for HepG2, MCF-7, HT-29 and A431 cells, respectively. Cell Cycle Analysis[1] Cell Line: H3255 cells Concentration: 1, 5, and 10 μM Incubation Time: 72 hours Result: Increased the percentage of H3255 cells in G0-G1 phase from 60.32% to 88.61%. Western Blot Analysis[1] Cell Line: H3255 cells Concentration: 0.039, 0.15, 0.62, 2.5, and 10 μM Incubation Time: 4 hours Result: Inhibited the phosphorylation of EGFR in H3255 cells in a dose-dependent manner.
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