Dansylcadaverine
Dansylcadaverine structure
|
Common Name | Dansylcadaverine | ||
|---|---|---|---|---|
| CAS Number | 10121-91-2 | Molecular Weight | 335.46400 | |
| Density | 1.19g/cm3 | Boiling Point | 505.5ºC at 760mmHg | |
| Molecular Formula | C17H25N3O2S | Melting Point | 137-140ºC | |
| MSDS | Chinese USA | Flash Point | 259.5ºC | |
| Symbol |
GHS07 |
Signal Word | Warning | |
Use of DansylcadaverineDansylcadaverine is a fluorescent probe. |
| Name | monodansylcadaverine |
|---|---|
| Synonym | More Synonyms |
| Description | Dansylcadaverine is a fluorescent probe. |
|---|---|
| Related Catalog | |
| In Vitro | Detection of Dansylcadaverine fluorescence after SDS-PAGE shows that the amount of actin-thymosin β4 crosslink is very low in the presence of Dansylcadaverine and transglutaminase, while there are large amounts of Dansylcadaverine-labeled thymosin β4 and actin. The amount of cross-link further decreases in the presence of Dansylcadaverine competing for Lys residues of either G-actin or thymosin β4 (100 μM). In the absence of thymosin β4, Dansylcadaverine-labeled actin polymerized to F-actin as revealed by fluorescence detection after native PAGE[1]. |
| Kinase Assay | To determine the time course of transglutamination, thymosin β4 (120 μM) is incubated with Dansylcadaverine (5 mM) in 70 μL buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl2, 3 mM DTT. The reaction is started by addition of 0.1 U transglutaminase. Immediately after addition of the enzyme (t=0) and at indicated times,10 μL are taken from the mixture, diluted in 490 μL 0.1% TFA to stop the reaction and analyzed by HPLC[1]. |
| Cell Assay | MCF7 cells (2.4×104) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope[2]. |
| References |
| Density | 1.19g/cm3 |
|---|---|
| Boiling Point | 505.5ºC at 760mmHg |
| Melting Point | 137-140ºC |
| Molecular Formula | C17H25N3O2S |
| Molecular Weight | 335.46400 |
| Flash Point | 259.5ºC |
| Exact Mass | 335.16700 |
| PSA | 83.81000 |
| LogP | 4.48510 |
| Vapour Pressure | 9.54E-11mmHg at 25°C |
| Index of Refraction | 1.594 |
| Symbol |
GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319-H335 |
| Precautionary Statements | P261-P305 + P351 + P338 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
| Hazard Codes | Xi: Irritant; |
| Risk Phrases | R36/37/38 |
| Safety Phrases | 26-36 |
| RIDADR | NONH for all modes of transport |
| HS Code | 2935009090 |
| HS Code | 2935009090 |
|---|---|
| Summary | 2935009090 other sulphonamides VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:6.5% General tariff:35.0% |
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| Dansylcadaverine |
| N-(5-aminopentyl)-5-(dimethylamino)naphthalene-1-sulfonamide |