5-Carboxyfluorescein diacetate N-succinimidyl ester

Modify Date: 2024-01-03 13:51:10

5-Carboxyfluorescein diacetate N-succinimidyl ester structure	Common Name	5-Carboxyfluorescein diacetate N-succinimidyl ester
	CAS Number	150347-59-4	Molecular Weight	557.461
	Density	1.6±0.1 g/cm3	Boiling Point	757.9±70.0 °C at 760 mmHg
	Molecular Formula	C₂₉H₁₉NO₁₁	Melting Point	152-154ºC(lit.)
	MSDS	USA	Flash Point	412.2±35.7 °C

Use of 5-Carboxyfluorescein diacetate N-succinimidyl ester

CFSE is a fluorescent dye which can track the cell division.

Name	5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein
Synonym	More Synonyms

Description	CFSE is a fluorescent dye which can track the cell division.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2].
Cell Assay	Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2].
References	[1]. Cinthia C. Stempin, et al. GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection. PLoS Negl Trop Dis. 2017 Jan; 11(1): e0005307. [2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.

Density	1.6±0.1 g/cm3
Boiling Point	757.9±70.0 °C at 760 mmHg
Melting Point	152-154ºC(lit.)
Molecular Formula	C₂₉H₁₉NO₁₁
Molecular Weight	557.461
Flash Point	412.2±35.7 °C
Exact Mass	557.095825
PSA	151.81000
LogP	0.50
Vapour Pressure	0.0±2.6 mmHg at 25°C
Index of Refraction	1.701
Storage condition	-20°C

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi
RIDADR	NONH for all modes of transport

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5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

Carboxyfluorescein diacetate succinimidyl ester

2,5-Pyrrolidinedione, 1-[[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]oxy]-

5(6)-CFDA (SE [5(6)-Carboxyfluorescein diacetate

MFCD00037466

5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester

5-{[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate

5-CFDA N-succinimidyl ester

5-Carboxyfluorescein diacetate N-succinimidyl ester

CFSE